ECP 2022 Abstract Book

Virchows Archiv (2022) 481 (Suppl 1):S1–S364 13 women > 30 years since 2018. The aim of this study is to analyse the detection of CIN2+ cases within the new screening protocol. Methods: HPV detection is performed by Cobas-HPV Test (Roche) with cytology as triage test. Colposcopy is indicated when cytol- ogy is positive (ASC-US or worse), and in HPV16 and/or 18 cases. Cross-referencing of the HPV screening database and the anatomic pathology database was performed, in order to obtain the CIN2+ detection rate. Results: HPV test was positive in 16.2 % (n=10,239) of women (range: 22.2% in 30-35 y.o, to 8 % in 51-65 y,). CIN2+ lesions were detected in: 15.9% of HPV16±others, 5.2% of HPV18±others and in 2.6% of non 16/18 HPV. Among HPV+ women 30 to 35 y.o., CIN2+ lesions were more frequent (23.4%) than in >35y.o. ones (18%). Conclusion: HPV based cervical cancer screening in our region has diminished more than 80% the number of cytologies, however more than 50% of them have abnormalities detected. The proto- col has shown a PPV of HPV for CIN2+ higher in HPV 16/18+ women (38 %) than in non-HPV16/18+ (14%). These results are in accordance with those in other countries and validate the new screening protocol. PS-11-023 Malignancy rate of atypia of undetermined significance/folli- cular lesion of undetermined significance in thyroid FNAs in Greater Vancouver, Canada R. Alaghehbandan*, J. Esmaeeli, T. Salisbury, L. Ali, S. Koonmee, O. Ondic, R. Bhan, K. Pivovarcikova, A. Gurung *University of British Columbia, Canada Background & objectives: Atypia of Undetermined Significance/ Follicular Lesion of Undetermined Significance (AUS/FLUS) is a challenging category comprised of a heterogeneous group of lesions. The objective of this study was to evaluate the malignancy rate of thyroid fine needle aspiration (FNA) diagnosed as AUS/ FLUS. Methods: This is a retrospective population-based study of all thyroid FNAs diagnosed as AUS/FLUS in Fraser Health in Greater Vancouver area during a six-year period (2014-2019). FNA diag- noses were correlated with clinical outcome in subsequent years including repeat FNA, surgery, and clinical/imaging follow-up. Clinical and radiologic factors were compared to identify malig- nancy-related features. Results: A total of 443 cases of AUS/FLUS were included. Repeat FNA was performed on 222/443 (50.1%) nodules, and 187/443 (42.2%) underwent surgery. The overall incidence of malignancy when a diagnosis AUS/FLUS is rendered in our study was 11.5% (51/443). Fifty one of 187 patients (27.3%) who underwent surgery had malignant thyroid carcinoma (38 papillary, 8 follicular, 5 with medullary, poorly-differentiated or anaplastic carcinoma). There were no statistically significant differences in age, sex and nodule size between benign and malignant cases. The rate of malignancy was 12.6% (28/222) in patients who underwent immediate sur- gery following the first AUS/FLUS diagnosis, while it was 10.4% (23/221) in patients who underwent repeat FNA (P=0.2). Conclusion: The malignancy rate of AUS/FLUS in the study is consistent with the recommended range proposed by the 2017 Bethesda System for Reporting Thyroid Cytopathology. Demo- graphic and radiologic findings were not significantly associated with upgrade malignancy risk. No significant difference was found in malignancy risk between those who underwent immediate sur- gery versus patients who underwent surgery after a repeat FNA following the initial diagnosis of AUS/FLUS. PS-11-024 Liquid-based cytology in the detection of premalignant lesions in patients with "Atypia in Squamous Cells" in conventional cytology Y. Vizcaino, L. Barrios, I. Benedetti* *Universidad de Cartagena, Colombia Background & objectives: Management of "Atypical Squamous Cells" (ASC) in conventional cytology (CC) is based on the risk of High-Grade Squamous Intraepithelial Lesion (HSIL). Efficacy of liquid-based cytology (LBC) to detect HSIL is variable, with little evidence of its performance in Colombian patients. Methods: Were obtained patients who attended colposcopy clinic due result of ASC in CC. A cervical sample for LBC was obtained from these patients which was interpreted by two pathologists with- out access to other results. The performance of LBC to detect HSIL was determined considering colposcopic/histological diagnosis as a gold standard: negative-satisfactory colposcopy/histopathological report. Two age groups were compared (<30/≥30 years). Results: Were included 114 patients, with previous report of ASC- US in CC, (there were no reports of ASC-H), with a mean age of 38.4 years (SD ± 13.3). LBC had abnormal results in 40.36% (n=46), with slightly higher proportion of Low-Grade Squamous Intraepithelial Lesion (LSIL) than HSIL. The total of abnormal diagnoses by colposcopy and/or biopsy was 51.75% (n=59) with a predominance of LSIL (36.84%). The sensitivity of the liquid- based cytology to detect premalignant lesions was 76.5%, specific- ity: 66.0%, positive predictive value: 28.3% and negative predictive value: 94.1%. The Cohen’s Kappa index of LBC for detecting HSIL was 0.2492 for the total population and 0.2907 for ≥ 30 years. Conclusion: This is the first prospective study conducted in Colombian patients with abnormal ASC cervical cytology to assess the diagnostic performance of LBC and CPS in detecting HSIL compared to histopathology. It can be concluded that although LBC decreases the total number of abnormal cytology and increases the detection of HSIL, improving diagnostic precision and decreasing the number of ASC-US, its concordance with the gold standard is discreet, being higher to detect HSIL especially in patients aged 30 years or older. Funding: Universidad de Cartagena, Cartagena, Colombia. PS-11-025 Applicability of the Sydney System and touch imprint cytology methodology in lymph node samples obtained by EBUS-TBNA. Three years of experience and 402 cases M. Berríos-Hernández*, H.E. Torres-Rivas, L.M. Fernández Fernández, M. Rubiera, M.d.l.P. González Gutiérrez, C. Fuente- Díaz, J.F. Pérez-Fontán, N. Zapico Ortiz, M. Ariza Prota, A.B. Dávila Lemos, M. Gil Muñiz, R. López González, J. Serrano Ortiz, K. Villar Zarra *Hospital Universitario Central de Asturias, Spain Background & objectives: The Sidney System for reporting lymph node FNA has standardized the diagnostic procedure. We present one of the few case series exploring the usefulness of this classification in EBUS-TBNA specimens. Methods: Over a 3-year period, EBUS-TBNA attended by the interventional pathologist were quantified. Patient demograph- ics, number of passes, and procedure time, among other variables, were recorded. A modification of the touch imprint cytology and ROSE methodology was used for on-site validation of the cell block sample. S128