ECP 2022 Abstract Book

Virchows Archiv (2022) 481 (Suppl 1):S1–S364 13 PS-17-001 Mature teratoma in the anterior mediastinum: a retrospective study of 34 cases O. Mnif*, R. Ayadi, E. Braham, M. Mlika, A. Rais, O. Ismail, A. Ayadi, F. El Mezni *Abderrahmen Mami Hospital, Tunisia Background & objectives: Mature mediastinal teratoma is a rare entity, accounting for approximately 8% of all tumours in this area. They are usually cystic benign tumours. The aim of this study was to describes the clinicopathological characteristics and discuss the dif- ferential diagnosis of this disease. Methods: We performed a retrospective study of 34 cases of mature mediastinal teratoma diagnosed at our department between 1992 and 2020. Results: There were 15 male and 19 female patients, aged between 5 and 61 years with a mean of 30,87. All patients underwent a surgical resection. On gross examination, mean size of the masses was 9,5 cm. The outer surface was smooth and tan-white, and the cut inner surface contained multiple cystic structures filled with a tanbrown substance and a strands of hair. Microscopy showed vari- able mature elements comprising of cysts lined by ciliated stratified epithelium and secretory epithelium, intestinal mucosa, sebaceous glands, smooth muscle, adipose tissue, abortive hair follicles, bone, cartilage, and pancreatic tissue. The cyst wall was fibrous with hya- linised areas. There was no evidence of immature, neuroepithelial elements. Outside the cyst, thymic remnants was identified (n=16) Conclusion: Mature mediastinal teratomas are rare tumours but should be considered in the differential diagnoses for mediastinal anterior lesion. Complete surgical resection is recommended in all mature teratomas with favourable survival rates. PS-18 | Poster Session Autopsy Pathology PS-18-001 Coinfection of HIV and COVID-19 V. Zinserling* *V.A. Almazov Research Center, Russia Background & objectives: Coincidence of HIV and COVID-19 is high. In spite of clinical publications depicting the prevalence of HIV and its complications, many items related to formulation of postmortem diagnosis and aspects of histopathology of lesions related to different pathogens remain unclear. Methods: We analysed all (148) autopsy cases where in clinical and/or pathological diagnosis were mentioned HIV and COVID-19 in the period from March 2020 till September 2021. Results: In the pathological diagnosis HIV was considered as main disease in 95 cases, COVID-19 - in 40 cases. In 9 cases other diseases were considered as main cause of death. In 4 cases new coronavirus infection was not included in pathological diagnosis. In majority of cases there were no difficulties in constructing the schema of tanatogenesis with the only exception when secondary infections in HIV were moderately expressed and lesions we con- sidered as manifestation of coronavirus infection were prominent. Most problematic were cases in which was present the combination with secondary pneumocystosis. Taking in consideration that inter- stitial lung fibrosis in both infections is similar special investiga- tion including immunohistochemistry is necessary. Conclusion: The rules of optimal formulation of pathological diag- nosis has to be discussed internationally, because of influence upon lethal statistics. Many aspects of relations between pathogens in the mixed infection needs further study. PS-18-002 Clinical and pathological data analysis in 73 deceased patients with previous SARS-CoV-2-associated pneumonia during con- valescence period L. Mikhaleva*, A. Kontorshchikov, V. Olesya, K. Maslenkina *A.P. Avtsyn Research Institute of Human Morphology, Russia Background & objectives: It is still not clear whether new corona- virus infection (COVID-19) has a long-lasting impact and can cause delayed sequela. The study aimed to analyse clinical and pathological data in patients who had previously had COVID-19 during early and late convalescence. Methods: We analysed medical history data and pathology find- ings of 73 deceased patients who survived COVID-19 and died from other causes in 2020-2021. The data included gender, age, data on previous coronavirus infection, disease duration, IgG and IgM antibody titers, CT scan results. We analysed initial and imme- diate causes of death in deceased convalescents of a new coronavi- rus infection were analysed. Results: Twenty six men and 47 women, aged from 33 to 104 years, were enrolled in the study. The antibody titer tests were performed at the time of hospital admission: the IgM level amounted from 0.31 to 1013.5 ОСЕ, IgG from 0.1 to 17.43 U/ml. The initial causes of death included circulatory system diseases in 63 patients (86.3%), digestive system diseases in 18 cases (24.7%), respiratory system diseases in 5 patients (6.9%). The cerebral oedema was considered as the immediate cause of death in 31 patients (42.5%), multiple organ failure in 13 cases (17.8%), heart failure in 13 patients (17.8%), endogenous intoxication in 6 patients (8.2%), other in 8 cases (13.7%). Conclusion: The period from clinical recovery to lethal outcome ranged from 2 to 300 days: 40 patients (54,8%) died after 2-30 days (early convalescents) and 33 (45,2%) after 31-300 days (late convalescents). Frequency of acute conditions of cardiovascular (acute myocardial infarction, bacterial endocarditis), respiratory (bacterial pneumonia) and cerebral (cerebral infarction, nontraumatic intracerebral haemorrhage) systems showed no difference in early and late convalescents (37,5% vs 33,3%, respectively, Ficher’s exact test: p > 0.05). Chronic disease progression prevailed in both subgroups of patients. PS-18-003 Splenic metastases in forensic pathology C. Amalinei*, A. Grigoras, L. Lozneanu, R.A. Balan, D. Ciobanu-Apostol *"Grigore T. Popa" University of Medicine and Pharmacy Iasi and Institute of Legal Medicine Iasi, Romania Background & objectives: Splenic metastases are relatively rare in forensics. The aim of our study was to highlight the features of splenic metastases from our files, in the context of widespread metastatic dis- ease in autopsy, using immunohistochemistry pattern to identify their origin. Methods: Necroptic examination and collection of specimens from six selected cases (37- 81 years old; equal distribution among genders), have been performed. Routine staining, along with a panel of immunohistochemical markers (Cytokeratin AE1/ AE3, CK5, CK7, CK20, CDX2, CD38, CD138, EMA, Cyclin D1, p53, p63, TTF-1, PSA, CEA, Synaptophysin, Chromogranin, and S100) have been used to to discriminate between different possible primaries. Results: The gross findings were that of multiple tumours with areas of necrosis. The microscopic examination of S154