ECP 2022 Abstract Book

Virchows Archiv (2022) 481 (Suppl 1):S1–S364 13 PS-19-007 Histological analysis of arteriovenous fistula specimens created for haemodialysis E. Horvath*, R. Kaller, B.A. Suciu, V.A. Mure ș an, M. Jakab, E.M. Arbanasi *George Emil Palade University of Medicine, Pharmacy, Sci- ence, and Technology of Targu Mures, Department of Pathology, Romania Background & objectives: Arteriovenous fistula (AFV) creation is the preferred access way for haemodialysis, but their functionality may be limited by pre-existing vascular pathology. In this study was analysed that the pre-existing vascular abnormalities represent a predictive value of AFV no maturation. Methods: Patients for chronic kidney disease (n=44) undergo- ing AFV placement were included in this study. The vein samples were processed for light microscopy, stained with H&E, trichrome, and von Kossa. The neovascularization and the chronic inflamma- tory cell population were analysed using, CD3 and CD68. Inti- mal hyperplasia and medial fibrosis were quantified using Image J analysis. Results: 45.45% of 44 biopsies showed concentric intimal hyperplasia, with a 46.2 μm mean by morphometric analysis. Medial fibrosis and expansion interested 54.54% of vein samples with 147.75 μm mean thickness, characterized by conjunctive fibres accumulation. Neoangiogenesis in intima and media layers only in 6.8% of cases was found. In vein walls, calcification and mononuclear inflammatory cells were absent. A subset of 9 specimens obtained from surgical revision patients had substantial luminal narrowing due to irregular neointimal formation (87.27 μm), media thickening (177.34 μm), and neoangiogenesis in intima and media. Foci of microcalcification and luminal thrombus were detected in 5 of 9 cases. Conclusion: In this study, we observed pre-existing abnormalities in both 9 cases, including neointimal hyperplasia disorganization of the venous wall and neovascularization of intima in veins used for AFV creation, that predispose these venous walls to maturation failure. The intima hyperplasia and neoangiogenesis in fistula with- out maturation are accompanied by mild inflammatory infiltrate, predominantly in those with thrombosis and calcification. PS-19-008 Primary heart tumours: a Portuguese case series V. Almeida*, G. Fontinha, B. Sepodes, J. Gama, V. Sousa, L. Carvalho *Centro Hospitalar e Universitário de Coimbra, Portugal Background & objectives: Primary heart tumours are rare, even in major cardiac surgery centres. We present a retrospective monocentric 5-year case series of resected primary heart tumours at Coimbra Hos- pital and University Centre. Methods: All firstly diagnosed primary heart tumours surgically resected at our Institution from 2017 to 2021 were retrieved from hospital registries. From each case, we collected patient age and sex, histopathological diagnosis, and intracardiac tumoral location. Follow-up time varied from 5 to 98 months. Results: We identified 34 tumours, 32 (94,1%) benign and 2 (5,9%) malignant. Regarding the benign tumours, we reported 24 (75%) myxomas and 8 (25%) papillary fibroelastomas. As expected, the majority (91,7%) of myxomas arose on the left atrium and papillary fibroe- lastomas on the valves. Twenty patients were female; the mean age was 62 years (range: 14-79). During follow-up, one myxoma recurred after eight months and was re-excised. No patient had significant complications nor died of the disease. The malignant tumours were an angiosarcoma and an EBV-positive diffuse large B-cell lymphoma, the first with a fatal outcome after six months. The patient with lymphoma completed chemotherapy and is well after 11 months. Conclusion: To the best of our knowledge, we describe the first Portuguese series on primary heart tumours. We report 94,1% of benign tumours, a significantly higher value than the 75% usually described in the literature (t(33) = -4,67, p < 0,001). We hypoth- esize that this difference is due to a higher incidental diagnosis of benign tumours, but further and more recent work is needed. We also report a myxoma recurrence, a rarely described event, and two uncommon malignant heart tumours. PS-19-009 Morphometric analysis of arterial wall main components densi- ties depending on the patient’s cause of death I.E. Plesea*, M. Serbanescu, D.A. Seicaru, M. Albu, F. Gherghi- ceanu, I.D. Avramescu, F. Giuroiu, O.C. Mirea, R.M. Plesea *”Carol Davila” University of Medici, Romania Background & objectives: Cardiovascular-CV diseases are among the most common causes of death. The authors compared the densities-D of aortic wall main components (Elastic fibres–FE, Collagen fibres- FCOL, smooth muscle fibres-FM) of people with CV and non- cardiovascular-NCV diseases causing patients’ death. Methods: Four aortic rings (base, cross, thoracic, abdominal) were taken during autopsies from 90 autopsied cases (62 NCV and 28 CV). Samples were processed using the classical HP tech- nique and stained with Orcein, and Goldner’s trichrome. Quan- titative measurements were made using custom-made software, developed in Matlab (Mathworks, USA) on virtual slides. Aver- age values were compared with “t” test and Pearson’s test. Results: FE-D and FCOL-D had a continuous descending trend in both CV and NCV groups in all main aortic regions. FE-D were higher in NCV group while FCOL-D were higher in CV group. In turn, FM-D had a general ascending but oscillating trend in both CV and NCV groups, more pronounced in the former. FCOL-Ds had a pronounced inverse correlation with both FE-Ds and FM-Ds and in both groups (correlation matrices-CMs negative and Pearson’s test “p” values <0,0001). In turn, FE-D had a direct correlation with FM-D in both NCV and CV groups, more pronounced in the former (CMs positive and Pearson’s test “p” value 0.0384 vs 0.0729). Conclusion: Our preliminary data show that the remodelling pro- cess of the aortic wall components differs between patients with CV and NCV diseases both along the aortic length and in the cor- relation pattern between the three aortic wall components (FE, FCOL and FM). PS-20 | Poster Session Electron Microscopy PS-20-001 Why do some uncemented porous tantalum total knee replace- ment fail? S. Fokter*, T. Bujas, Ž. Ledinek, J. Zajc, N. Gubeljak, E. Punzón-Quijorna *University Medical Centre Maribor, Slovenia S158