ECP 2022 Abstract Book

Virchows Archiv (2022) 481 (Suppl 1):S1–S364 13 rates of relapse and/or persistence of disease) compared with other groups (p<0.001). In addition, despite no statistically significant, the tumour size of VSCC associated to HSIL-like lesions was smaller than of other groups (p=0.17). No differ- ences in overall or disease-specific survival were identified among the six groups (p=0.15). None of the women with VAAD or DEVIL died of disease. Conclusion: Our findings indicate higher risk of relapse or per- sistence of disease in patients with VSCC arising on HSIL-like or VAAD/DEVIL lesions. Closer surveillance after surgical resection of VSCC may be indicated for these cases. Funding: Project “PI20/00368; Caracterización genómica de los carcinomas de vulva independientes de virus del papiloma humano y de sus precursores”, funded by Instituto de Salud Carlos III and co-funded by the European Union (ERDF) “A way to make Europe”. ISGlobal receives support from the Spanish Ministry of Science and Innovation through the “Centro de Excelencia Severo Ochoa 2019-2023” Program (CEX2018-000806-S), and support from the Generalitat de Catalunya through the CERCA Program. OFP-02-010 Reproducibility of low-volume lymph node metastasis assess- ment in endometrial cancer M. Shahi*, D. Sadeghian, A. Yilmaz Altay, P. Biagio, M. Ghioni, G. Mehmet, D. Fanni, J. Bennett, E. Unti, J. Cheville, A. Larish *Mayo Clinic, Rochester, MN, USA Background & objectives: Lymph node (LN) assessment is criti- cal for staging of endometrial cancer (EC). Low volume metastasis (LVM), upstages patients, but early data suggests some of these patients can be spared adjuvant treatment. So, accu- rate pathologic determination of LVM is critical. Methods: Whole slide images (WSI) (3 H&E levels, 40 μm apart and 1 CK AE1/AE3 slide) of 11 pelvic sentinel lymph nodes (SLN) involved by LVM were reviewed by 9 pathologists from multiple institutions. Various parameters were assessed: number of foci, size of the largest focus, clusters versus scattered cell, site of involve- ment in the LN and extra-nodal extension. Results: An international consortium of 9 pathologists were participated in this study. 9 out of 11 cases, ≥8 pathologists had complete agreement determining the type of involvement (MM vs ITC). In the remaining 2 cases, there was an even split in the designation of the involvement type. These two cases showed multiple scattered tumour foci with dispersed cells, making the accurate measurement and determination of the involve- ment type challenging. The largest focus measurements showed some disagreement in both micro-metastasis and ITC cases, with slightly higher variability in the ITC sub-group. Agree- ment in #foci/plane, pattern, and site of involvement were high. Conclusion: Our study indicates an excellent interobserver agree- ment for the separation of ITCs and MM amongst an international group of gynaecologic pathologists using WSI. Problematic cases arise when tumour foci extend along a distance within a lymph node. Additional studies are needed to determine the impact of these findings and to formulate recommendations to address. OFP-02-011 Evaluation of the implementation and diagnostic accuracy of the Paris classification for reporting urinary cytology in voided urine specimens: a cyto-histological correlation study in a high- volume cancer centre J. Lobo*, C. Lobo, L. Leça, Â. Rodrigues, R. Henrique, P. Monteiro *IPO Porto, Portugal Background & objectives: The Paris classification was introduced for reporting urinary cytology, highlighting the need to focus on accurately identifying high-grade urothelial carcinoma (HGUC), and aiming to improve criteria, terminology and ultimately improv- ing patient management. Methods: To assess the overall implementation and diagnos- tic performance of the Paris classification for reporting urinary cytology. All urinary cytology reports from July 2018-December 2019 were collected (n=1240). Only voided urine samples were included (n=1180), of which 9.9% had histological confirmation. Risk of malignancy (ROM) was calculated. Diagnostic perfor- mance of urinary cytology was assessed, including sensitivity, specificity, PPV, NPV and accuracy. Results: The median age of the study population was 69 years, and 71.2% were male. The Paris system categories were widely used (in 99.7% of reports). The distribution of categories was: 0.3% unsat- isfactory, 90.5% negative for HGUC, 5.6% atypical urothelial cells (AUC), 1.6% suspicious for HGUC, 1.9% HGUC and 0.1% other malignancies. No diagnosis of low-grade urothelial neoplasia was given. ROM was 21.4% for negative for HGUC, 66.7% for AUC, 91.7% for suspicious for HGUC and 100% for HGUC. When using suspicious for HGUC as a cut-off, the diagnostic performance of urinary cytology in identifying HGUC was 46% sensitivity, 98% specificity, 96% PPV, 68% NPV and 74% accuracy. Conclusion: Specificity of urinary cytology is very high. ROM for each category was in accordance with literature, except for AUC where ROM was slightly higher (66.7%). Sensitivity of voided urine specimens is known to be lower than that of instru- mented specimens, explaining the lower sensitivity in our study. Study population characteristics (high-volume cancer centre with many patients treated with intra-vesical therapies) may explain part of our results. This pilot study motivated an inter- observer variability study among three Cytopathologists, which is ongoing. OFP-02-012 Application of a standardised terminology and nomenclature for respiratory cytology: experience from a large tertiary res- piratory cancer centre D. O’Connor*, A. Fabre, M. Mc Nally, D. Gibbons *St Vincent’s University Hospital, Ireland Background & objectives: International cytopathology coding systems have a valuable role in facilitating report standardisation. A new international respiratory cytopathology coding system is currently being developed. Our institution has been coding all res- piratory cytology specimens in a similar manner for over 10 years. Methods: Specimens are coded as non-diagnostic (C1), benign (C2), atypical, favour reactive (C3), suspicious for malignancy (C4) or malignant (C5). We calculated rates of diagnostic categories on our cohort over a two-year period by evaluating all endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA), bronchial wash- ing, bronchial brushing, bronchial lavage and sputum specimens performed at our institution using the laboratory information system. Results: We assessed all aforementioned respiratory cytological specimens received at our institution in the years 2020 and 2021. In total, 1433 cytological specimens fulfilled the inclusion crite- ria and were analysed. Of these, 15.8 % (n=226) were coded as malignant (C5) and 1.47% (n=21) were coded as suspicious for malignancy (C4). The calculated rates of diagnostic categories, bases on cytological findings, were as follows: S9