ECP 2022 Abstract Book

Virchows Archiv (2022) 481 (Suppl 1):S1–S364 13 Background & objectives: To understand the morphogenesis of breast cancers and non-malignant breast diseases, it is necessary to analyse the reorganization of two structurally and functionally interconnected tissue components such as parenchyma and stroma. Methods: We present an analysis of the tumour extracellular matrix of 5 cases of invasive ductal carcinoma of no special type (IDC NST). The subject of the study was postoperative samples of 5 breasts obtained from sectoral resection with lymph node dissec- tion, for IDC NST. Histological study, IHC-study and transmission electrone microscopy was carried out. Results: As a result of the study, it was found that the extracellular matrix of the IDC NST stroma contains a large number of thin and thick collagen fibrils concentrated in the perivascular tissue and in the foci of the desmoplastic reaction. Quite often, basement membranes are adjacent to the cytoplasm of carcinoma cells. In some foci, we found striated collagen fibres, as well as microfibrils shaped like an electron-dense rod. In most cases, the perivascu- lar tissue resembled connective tissue containing sparse collagen fibres with fragments of proliferating vessels, areas of necrosis, and cells that can be regarded as fibroblasts and macrophages. Interest- ing that we didn’t find pericytes in the capillaries. Conclusion: Changes in the stroma during tumour progression consist in a decrease in the total amount of the non-fibrillar matrix in the background of increase in the amount of collagen and elastic fibres, and the appearance of lymphoplasmacytic infiltrates. Changes in the histoarchitectonics of the microcirculation are an integral part of tumour progression. Extracellular matrix is characterized by an increase in the number of pericyteless capillaries localized mainly in the periphery of the tumour and directed outward from the tumour. E-PS-02-037 Axillary region nodule: an unusual finding Y. Fejji*, N. Abdessayed, Z. Lajmi, A. Bchir, S. Yacoub, M. Mokni *Department of pathology, Farhat Hached University Hospital, Sousse, Tunisia Background & objectives: Ectopic breast tissue in the axillary region can give rise to tumoral conditions which are challenging to diagnose. This study aims to present a case of fibroadenoma (FA)of the axillary region revealing an ectopic breast tissue, mimicking an adnexal gland tumour clinically. Methods: A 25-year-old female without past medical history pre- sented with a mass in her left axilla evolving for 4 years. On clini- cal examination, the mass was firm, freely mobile, and completely isolated from the left breast. Ultrasound showed a solid, oval, het- erogeneous, hypoechoic node measuring 2*1cm. The ultrasound- mammogram was normal. The mass was excised and addressed for a histopathology examination Results: On gross examination, the mass was firm with the bos- selated surface, whitish. The serial sectioning was homogeneous. Histological examination showed that the mass was composed of 2 components: epithelial and mesenchymal. The mesenchymal component is made of hyaline connective tissue with uniform cel- lularity. The epithelial component is made of stretched ducts lined by a double cellular layer without atypia. The diagnosis of fibroad- enoma in the axillary accessory breast was retained. Conclusion: A mass of the axillary region is a common clinical presentation. The most frequent diagnoses are lymph node malignancy followed by sweat gland tumours. FA in the axillary region is a rare disorder representing a diagnostic dilemma. confused with other benign or malignant pathologies. It most commonly affects adult women aged in the 3rd and 4th decade of life. Imaging is not contributive. Pathological examination is mandatory to rule out another differential diagnosis, especially malignancies. E-PS-02-038 A rare case of metaplastic carcinoma with neuroendocrine differentiation S. Bakhiet*, A. Behzadnia, A. Elhag *Hull University Hospitals NHS Trust, United Kingdom Background & objectives: Metaplastic breast carcinomas represent a heterogeneous group of carcinomas with epithelial and mesenchymal components. Primary neuroendocrine breast carcinoma is an exceed- ingly rare entity. Here we describe the first reported case of metaplastic breast carcinoma with neuroendocrine differentiation. Methods: A 60-year-old lady was found to have a suspicious nodule in her left breast, measuring 19mm on radiology. Following the core biopsy, a diagnosis of malignancy was made, and she proceeded to have a wide local excision and sentinel lymph node biopsy. Results: Histological examination of the specimen showed an infiltrative solid tumour, composed of predominantly tightly packed small basophilic cells, with stippled nuclear chromatin, inconspicuous nucleoli growing in sheets, with large areas of necrosis and apoptotic debris. Admixed with these undifferentiated neoplastic blue cells were small clusters of epithelioid groups showing squamous differentiation. The undifferentiated blue cells showed a neuroendocrine carcinoma phenotype with AE1/3, CK7, CD56 and synaptophysin positivity as well as basal marker positivity (CK5/6, p63 and CK14). The epithelioid component was also AE1/3, CK7 and CAM5.2 positive with no neuroendocrine differentiation. Based on the immunomorphological features a diagnosis of triple-negative metaplastic carcinoma with neuroendocrine differentiation was made. Conclusion: Metaplastic carcinomas have monomorphic or bipha- sic epithelial and mesenchymal components. Identifying in-situ components can aid the diagnosis, as in this case. Primary neu- roendocrine tumour of the breast is an extremely rare entity, that requires clinicoradiological correlation to exclude metastasis, and in view of this case, may also require exclusion of metaplastic carcinoma with neuroendocrine differentiation. E-PS-03 | E-Posters Cardiovascular Pathology E-PS-03-002 New perspectives in cardiac surgery: electrical DC tensions implements morphologic patterning in chick heart embryo model A. Capuani* *Private Organization Carrara, Italy Background & objectives: Cell polarity and electrotaxis play an important role in cardiac embryogenesis and on genes expression to specific phenotypes. Chemical and physical electrodynamic laws direct the bioelectric endogenous fields. (Faraday 1831, Henry 1832, Maxwell 1865, Wolpert 2015, Levin 2017). Methods: We designed devices A and B: A- A passive two terminal condenser storing an uniform 800 mV / mm electric field. Inside we accommodated 30 fertilized eggs for 52 hours after laying (gastrulation, looping, septation) inverting the polarity. B- A second device /same Electrical Field, to treat 7 fertilized cockerels during the egg passage in the oviduct (germinal disk, blastula, gastrula). Results: In group A we observed 4 giant Omphaloceles (18 %). Overall mortality 13.6% (Capuani et al Virchows 2020). In group S201