ECP 2022 Abstract Book

Virchows Archiv (2022) 481 (Suppl 1):S1–S364 13 E-PS-09-048 Mucinous borderline ovarian tumours: challenging diagnostic A. Fitouri*, G. Sahraoui, F. Ben daoued, O. Jaidane, L. Charfi, H. Arbi, H. Azaiez, N. Nassraoui, N. Boujelbene, I. Abbes, K. Mrad, R. Doghri *Pathology Department, Salah Azaiez Institute, Tunis, Tunisia; Research Laboratory LR21SP01, Tunis, Tunisia Background & objectives: Ovarian mucinous borderline tumours (MBT) are characterized by an epithelial proliferation similar to those of well differentiated adenocarcinomas but are distinguished by the absence of stromal invasion. The aim of the work was to specify the pathological and clinical features. Methods: Retrospective study including 49 cases of primary ovar- ian MBT, diagnosed at the Patholgy Department of Salah Azaiez Institute from 1992 to 2019. We included in our study all patients who presented with a primary MBT on a surgical specimen. The collection of clinical data was made from the medical records. Results: Median age was 48 years old. Histologically, the cases were divided into 34 cases of pure MBT, 13 cases with intraepithelial carcinoma and 2 cases associating an intraepithelial carcinoma with microinvasion. The majority of our cases were classified FIGO I and only one case FIGO III. Sixteen patients received conservative treatment and 30 received radical treatment. The treatment wasn’t specified in three patients. The prognosis was good in the majority of cases. Only one patient had a contralateral recurrence after a follow-up period of three years. Conclusion: The diagnosis of MBT is difficult. Indeed, the distinction of MBT from carcinomas remains the greatest challenge for pathologists. Once this diagnosis is made with certainty, the tumour can be considered to have a good prognosis, especially stage I tumours which are the most common. Prospective and multicentre studies would be necessary for a better understanding of these tumours and their evolution. E-PS-09-049 The role of eIF signalling in benign proliferative disorders of the endometrium T.S. Driva*, M. Sobočan, C. Schatz, J. Haybaeck *First Department of Pathology, Medical School, National and Kapodistrian University of Athens, Greece Background & objectives: Adenomyosis, endometriosis and typical endometrial hyperplasia are common benign proliferative disorders of the endometrium that afflict many women with life-impacting conse- quences. The aim of this analysis was to explore the impact of different translational markers on non-malignant endometrial diseases. Methods: We assessed evidence on the expression of eukaryotic translation initiation factors (eIFs) in adenomyosis, endometriosis and typical endometrial hyperplasia compared to their expression in normal endometrium. We analysed the impact of deranged eIF expression on endometrial function and pathogenesis of non-malig- nant neoplastic endometrial disorders. This database analysis was performed through PubMed and Google Scholar. Results: Adenomyosis is characterized by dysregulation of eIF2 and eIF4 signalling. The factors eIF4a2, eIF3K and eIF4b are expressed differently between adenomyotic and normal endome- trium. Furthermore, decreased expression of eIF3e in adenomyosis and ovarian endometriosis tissue has been implied to promote EMT in these conditions via TGF-β1 or Snail activation. In addition, eIF2α signalling can serve as a treatment target for endometrio- sis. Specifically, the progestin medication dienogest as well as the flavonoids naringenin and chrysin exhibit a suppressive role in endometriotic cell lines by activating eIF2α and thus enhancing endoplasmic reticulum stress. Moreover, eIF2α signalling seems to be involved in the pathogenesis of typical endometrial hyperplasia in PCOS. Conclusion: eIF signalling is dysregulated in adenomyosis, endo- metriosis and typical endometrial hyperplasia. The derangement of eIF2, eIF3 and eIF4 expression seems to contribute to the develop- ment of these benign endometrial conditions and those eIFs may serve as druggable targets for these life-impacting diseases. E-PS-09-050 Collision tumour: a rare case report B. Simsek*, E. Atik *Mustafa Kemal University, Turkey Background & objectives: Collision tumours are composed of two histologically distinct neoplasm in the same organ without intermix- ture of cell types. We present here the case of a 79 years female with bilateral high grade serous carcinoma with mature cystic teratoma. Methods: Microscopic examination revealed hematoxylin sections of the right and left ovaries, with cystic structure containing skin joints keratin hair follicles, tumoral islands with bizarre nuclei in large necrosis areas Bilateral fallopian tubes also showed high grade serous carcinoma. In the immunohistochemical study, tumour cells were stained positive with CK 7P53 and negative with CK20 CDX2. Ki 67 proliferation index is 90%. Results: Collision tumour containing bilateral serous carcinoma and mature cystic teratoma component is very rare entity. Conclusion: Collision tumour containing bilateral serous carci- noma and mature cystic teratoma component is very rare entity. E-PS-09-051 High-grade endometrial carcinomas: diagnostic challenges G. Sahraoui*, A. Maaoui, R. Doghri, N. Kouki, L. Charfi, N. Bou- jelben, I. Abess, M. Ghaleb, R. Mchiri, N. Ben Hamida, K. Mrad *Pathology Department, Salah Azaiez Institute, Research Labora- tory LR21SP01, Tunis, Tunisia Background & objectives: The distinction of certain histologic sub- types of high grade carcinomas is not uncommonly problematic, and as such, immunohistochemical study is often needed. We performed an overview of the histologic and immunohistochemical features of the different subtypes of high-grade endometrial carcinomas. Methods: Retrospective study of 26 high-grade endometrial carcino- mas collected in the pathology laboratory of Salah Azaiez Institute in Tunis over a period of 20 years. We selected the cases of high-grade endometrial carcinomas that couldn’t be classified based only on the histopathology and required complementary immunohistochemistry. We performed clinicopathology data collection which involved age, tumour size, histologic subtype and immunohistochemical data. Results: The average age was 63 years old. Endometrioid carcinoma grade 3 represent 23%, serous carcinoma 27%, clear cell carcinoma 8%, carcinosarcoma 19% and 23% of cases were classified as high grade carcinoma. The most frequent immunohistochemical profile for endometrioid carcinoma grade 3 is strong positivity for ER/PR, negativity to patchy positivity for p16, and wild-type p53 staining pattern. Serous carcinomas were mutation-type p53 staining, ER and PR variable postivity and strong p16 positivity. Clear cell carcinomas were negative for ER, RP, p16 and p53. Carcinosarcoma profile was focal CD10 staining and negativity of cytokeratin, vimentine and caldesmone. Immunohistochemistry was inconclusive in five cases classified as high grade carcinoma. S269