ECP 2022 Abstract Book

Virchows Archiv (2022) 481 (Suppl 1):S1–S364 13 urinary tract. 4–17% of the cases develop urethral recurrence after cystectomy affecting the prostatic gland either by invasion or by pagetoid extension through the prostatic duct. Methods: A patient 70-years-old was admitted to our hospital with a previous prostate needle biopsy, which on histopathologi- cal examination showed urothelial carcinoma extending into the prostatic ducts. We review the biopsy and proceed to bladder biopsy from different sites. A biopsy of prostatic urethra was made too. After the pathology results a radical cystoprostatectomy was decided. Results: All urinary bladder biopsies showed in situ urothelial carcinoma. In the specimen of radical cystoprostatectomy, after extensive sampling, the histopathology examination showed in situ urothelial carcinoma which involved prostatic ducts and glands in a pagetoid manner, without invasive component. The same lesions were recognized in both ureters. The seminal vesicles, the pros- tatic urethra margin and both ureteral margins were negative for in situ or invasive carcinoma. All ten lymph nodes were negative for metastatic carcinoma. The immunohistochemistry was positive for GATA-3, p63, CK20 and negative for PSAP and PSA. The p63 and CK14 shows pres- ence of myoepithelial cells surrounding prostatic ducts and glands. Conclusion: Conservatory treatment is an option for in situ urothelial carcinoma extending in the prostatic duct. Radical surgery is the best treatment for extensive intraductal prostatic involvement. The clinicopathologic features associated with an increased risk of urethral recurrence are: involvement of the urinary bladder neck or trigone, diffuse carcinoma in situ, multifocality, synchronous upper urinary tract tumours, involvement of the prostatic urethra or deep prostatic invasion and the positive urethral margins. E-PS-24-015 Seminoma in a case of ovotesticular disease (true Hermaphrodith) S. Cagaptay*, C. Altay, G. Aslan, K. Yorukoglu *Dokuz Eylül University Faculty of Medicine, Turkey Background & objectives: Ovotesticular disorder is defined as the presence of both ovaries and testes in the same person, regardless of karyotype. Patients with ovotesticular disorder have a higher risk of developing gonadal neoplasms such as gonadoblastoma or seminoma than the general population. Methods: The karyotype analysis of a 32-year-old male patient with phenotype who was examined for bilateral abdominal unde- scended testis was 46 XY. In his radiological examination, a complex internal genital structure compatible with ovotestis was detected. The patient whose operation material was examined in our unit is presented with histopathological and clinical features. Results: Macroscopically, the specimen consisted of bilobed gonadal structures and a single cordial structure adjacent to it. In the histopatho- logical examination, in addition to seminoma, tissues including vesicle seminalis, atrophic testis, tuba uterina, endometrium and endocervix component were seen. Most of the structures thought to belong to the female genital tract were not of the usual morphology, possibly due to insufficient hormonal effect. Immunohistochemical markers contrib- uted greatly to the distinction of the organs and diagnosis. The patient, who was diagnosed with ovotestis and Stage I seminoma based on histopathological findings, received a single dose of carboplatin chemo- therapy and free of disease at 6th month of follow-up. Conclusion: Ovostesticular disorder is a very rare sex develop- mental disorder, with a 10% risk of germ cell tumour develop- ment in 46XY and mosaicism cases. As a result, orchiectomy is required in the presence of an unde- scended testis or development of a tumour. Because male and female genital organs have similar morphological and immuno- histochemical features, a multidisciplinary approach is required for pathological examination. E-PS-24-017 Old markers, new perspective in urothelial carcinoma L. Cristina*, O. Stefan, G. Tudor, V. Nica, V. Mitroi, L. Nichita *Colentina Clinical Hospital, Romania Background & objectives: Bladder cancer is one of most common malignancies of the urinary tract. The present study was conducted to study the immunoexpression of CD10, c-KIT and Her2 protein in urothelial carcinoma and to correlate it with histological grade and pathological stage. Methods: 41patients were subjected TURBT for non-muscle- invasive and muscle-invasive urothelial carcinoma between 2019-2021 in our hospital.CD10, HER2 and c-KIT membranous/ cytoplasmic expressions in cells were the main variables of interest; strong-positive result is defined as strong/moderate immunoreactivity in >50% cells(2+), weak-positive result is defined as mild immunoreactivity of <50% of tumour cells(1+) and cells with faint/no staining will be scored as negative(0). Results: High-grade T2 tumours showed strong or week expression of all the markers that we used: HER2 staining was graded 2+ in 2/8 high-grade T2 tumours (25%) and 1+ in 3/8 high-grade T2 tumours (37,5%). Strong and week immunoreactivity of c-KIT was noted in 12,5% respectively 25% in this category of tumours. 50% (4/8 high-grade T2 tumours) showed strong membranous staining for CD10. Regarding of Ta and T1 high-grade tumours, week expression of HER2 (4/25 tumours), c-KIT (5/25 tumours) and CD10 (6/25 tumours) was observed. All low-grade Ta and T1 tumours were negative. Conclusion: Our findings indicate that overexpression of HER2 protein, expression of CD10 and c-KIT is correlated with high- grade tumours and stage, suggesting its possible role in patho- genesis and progression of urothelial carcinoma. E-PS-24-018 A bone in the bladder – rare case report and review of literature O. Stefan*, G. Tudor, L. Cristina, A. Toma, V. Mitroi, M. Cio- plea, L. Nichita *Colentina Clinical Hospital, Romania Background & objectives: Stromal osseous metaplasia (SOM) is one of the three instances of bone formation within bladder tumours. It is also the least common, with a total of 21 cases reported in the English literature. Here, we report an additional case. Methods: A 64-year-old man with a history of multiple acute cystitis presented to our hospital with urinary retention. Cys- toscopy revealed a 3 cm pedunculated tumour with papillary architecture located on the right lateral bladder wall, removed with transurethral resection. Results: Pathological examination was performed on pink-tan to grey friable tissue fragments that varied from soft and gelatinous to firm. Microscopy showed papillary projections lined by a thick layer of stratified transitional epithelial cells with central fibro- vascular cores, with orderly architecture and mild pleomorphism. Invasion through the basement membrane was not identified. S353