ECP 2023 Abstracts

S179 Virchows Archiv (2023) 483 (Suppl 1):S1–S391 13 for distinguishing acantholytic squamous cell carcinoma from other mimics. E-PS-04-018 A cytohistological correlation study: cytological traits associated with cervical adenocarcinoma M. Veras*, A. Díez Fernández, E. Revilla Sánchez, N. Alberti Masgrau, A. Pérez Campos *Pathology Department, Hospital Universitario 12 de Octubre. Madrid, Spain Background & objectives: The increasing incidence of cervical ade- nocarcinoma compared to squamous carcinoma suggests that cervical screening is inefficient at preventing it. The objective of this study is to analyse the cytological features associated with cervical adenocar- cinoma and their histological correlation. Methods: Cytology specimens from women with concurrent, histo- logically confirmed cervical adenocarcinomas/in situ (AIS) from 2017 to 2022, and cytology slides with a result of atypical glandular cells (ACG) from the same period and a follow-up biopsy, were reviewed retrospectively for cytologic traits associated with adenocarcinomas. Cytohistological correlation was studied. Clinical data and HPV status were also analysed. Results: 26 cases diagnosed as cervical adenocarcinoma in biopsy were selected. Average age was 50.6 years. 65.4% (n=17) of cases with an available HPV test result were HPV-positive, all for high-risk genotypes. 19.2% (n=5) were identified cytologically as adenocarcinomas/AIS. 50% (n=13) fell into a diagnostic group that implied follow-up biopsy, that is, adenocarcinomas/AIS and high-grade lesions (HSIL/ASC-H/ AGC). Also, histologically, of 128 cases of ACG, 27.34% (n=35) were high-grade lesions, and 8.59% (n=11), adenocarcinomas/AIS. Cytologic traits associated with adenocarcinomas will be reviewed. Conclusion: A substantial proportion of cases with histologically con- firmed cervical adenocarcinomas do not receive a cytological diagno- sis of adenocarcinoma or other high-grade lesion that assures a histo- logical follow-up, and consequently, diagnosis and treatment of these cases might be delayed. Most cases of AGC do not correspond with malignant or premalignant lesions. These results indicate that cytology screening is not efficient at detecting cervical adenocarcinomas, and a better definition of diagnostic criteria is needed. These findings should be consolidated in future studies. E-PS-04-019 HPV test in cytology laboratory practice: a six-year follow-up study D. Versa Ostojic*, D. Vrdoljak-Mozetic, S. Stemberger-Papic, M. Dinter, R. Rubesa-Mihaljevic *Department of Pathology and Cytology, Clinical Hospital Centre Rijeka, Croatia Background & objectives: Cervical cytology and HPV test are tools in the prevention of cervical cancer. The aim of the study was to com- pare the initial cytology and HPV test with the detection of HSIL+ histology and triage of ASCUS in six-year follow-up. Methods: We analysed retrospectively the results of 1157 HPV tests (Hybrid Capture 2) and initial cytology in the detection of cervical intraepithelial neoplasia or in follow-up after excisional treatment. The results were compared with the outcome after six-year follow-up period. Negative follow-up cytology or negative histology was consid- ered a negative outcome. HSIL+ histology was considered as a positive outcome. Results: 473(40.9%) HPV tests were positive and 652(56.4%) had abnormal cytology (mean age 37 years, range 16-77). In the six-year follow up period 173 of 213 patients had HSIL+ histology. The false negative rate of HPV test for HSIL+ was 1.8%. HSIL+ was found in 2.5% of patients with initially negative cytology, 8.3% of ASCUS, 10.9% of LSIL, 10% of AGC, 56.5% of ASC-H, 63.5% of HSIL and in 100% of cytologically detected cancers. The HPV test showed 94.2% sensitivity, 68.4% specificity, 34% positive predictive value (PPV) and 98.5% negative predictive value (NPV). The reflex HPV test in ASCUS triage showed 86.2% sensitivity, 65.9% specificity, 18.7% PPV and 98.1% NPV. Conclusion: The HSIL+ histology correlates with the severity of cyto- logical diagnosis. High NPV of HPV test is shown to be a valuable tool for triaging patients with ASCUS. Although the HPV test exhibits high sensitivity and NPV, its moderate specificity and PPV suggest it should be used in conjunction with cytology. Regular screening and follow-up after treatment of HSIL are crucial for the prevention of cervical cancer E-PS-05 | E-Posters Dermatopathology E-PS-05-001 Contribution of direct immunofluorescence in the diagnosis of inflammatory dermatoses: a Moroccan study G. Ablouh*, K. Znati *Mohammed V University - Faculty of Medicine and Pharmacy of Rabat, Morocco Background & objectives: The use of direct immunofluorescence (DIF) has enhanced the diagnosis of inflammatory skin conditions by identifying target antigens with specific antibodies on frozen biopsy samples. This study aims to investigate the diagnostic value of DIF in Morocco for Inflammatory Dermatoses. Methods: We conducted a retrospective cohort study at the Anatomic Pathology Laboratory of Avicenna Hospital in Rabat. Our sample consisted of 30 patients who underwent a direct immunofluorescence (DIF) test in dermatology between 2015 and 2016. By analyzing patient records, we aimed to investigate the relationship between DIF findings and dermatological diagnoses. Results: The study examined 30 patients who underwent direct immu- nofluorescence (DIF) testing for inflammatory dermatoses between 2015 and 2016. The final diagnoses for inflammatory dermatoses with positive DIF are as follows: four patients were diagnosed with pemphi- gus (including one deep and three superficial pemphigus), two patients were diagnosed with bullous pemphigoid, and two patients were diag- nosed with lupus erythematosus. The study revealed a positive DIF value of 66% in pemphigus, 40% in bullous pemphigoid, and 30% in lupus erythematosus. Conclusion: Our investigation demonstrated that the diagnostic value of DIF in inflammatory dermatoses is dependent on the type of dermatosis. The utilization of DIF in Morocco is below the interna- tional standard, indicating the necessity for more precise guidelines to increase diagnostic accuracy while minimizing costs. However, due to limited data, our study had a small sample size. Hence, a more exten- sive study is required to establish more robust conclusions. E-PS-05-002 Primary cutaneous cribriform apocrine carcinoma. A case report L. Aguirrezabal Marcotegui*, S. Fernández Ferrer, A. Tarín Nieto, J. Roselló Soria, I. Fernández de la Prieta, C. Valentí Ponsa, G. Cancho Galán, A. Nogueira Gegrorio *Hospital Universitario de Basurto, Bilbao, Spain Background & objectives: Primary cutaneous cribriform carcinoma (PCCC) is a rare histopathological variant of apocrine carcinoma of the skin that clinically presents as a solitary nodule located on upper and lower extremities. We present the case of a patient with PCCC. Methods: A 78-year-old man with past medical history remarkable for hypertension, degenerative dementia and adenomectomy for benign prostatic hyperplasia, presented a nodule in the right groin of several