ECP 2023 Abstracts

S298 Virchows Archiv (2023) 483 (Suppl 1):S1–S391 13 Conclusion: The dynamics of the levels of MMP2, MMP8 and MMP9 expression suggests the effectiveness of the new protocol, also as com- pared to the standard therapy. At the same time, wave-like dynamics of MMP1 and MMP14 expression correlated with clinical signs of relapse and indicated their role in the progression of inflammation and destruction of periodontal tissues with insignificant response to the applied therapy. E-PS-13 | E-Posters History of Pathology E-PS-13-001 NGS evaluation of an ovarian neoplasm of a 19th century natural mummy from Goriano Valli, Central Italy C. Bellevicine*, F. Pepe, C. De Luca, C. Mercurio, A. Avitabile, M. Traversari, L. Ventura *Università Federico II Department of Public Health, Italy Background & objectives: The body of an anonymous female dating back to 19th century was found among the natural mummies in the friary of “San Giorgio degli Osservanti” in Goriano Valli, L’Aquila, central Italy. A paleoradiologic, paleopathologic and molecular study was undertaken. Methods: Autopsy study performed by posterior approach and CT were performed. Samples of the organs were rehydrated using Sandison solution, fixed in formalin, and embedded in paraffin to obtain sections stained with H&E. DNA was extracted and its quantity (6.63 ng/μL) and quality (DIN=1.3) was evaluated by Genomic DNA ScreenTape assay. Oncomin BRCA Assay GX on NGS platform (Thermofisher) was performed. Results: The body (stature: 166+/-4 cm; weight: 4,050 g) belonged to a 43–50 years old woman affected by severe periodontitis, lung anthracosis, diffuse pleural adhesions related to pleuritis, abdominal distension, and perivescical phleboliths. CT examination showed a large cribrous mass in the right iliac fossa and pelvis. Histology showed a cystic-solid architecture, possibly related to an ovarian neo- plasm. NGS analysis revealed a BRCA2 Variant of Uncertain Sig- nificance (VUS) (c.7093C>T, p.His2365Tyr), whose Mutant Allelic Fraction (MAF) was 21.8%. Secondary level analysis by using Golden Helix Genome Browser v.2.0.7 (Bozeman, MT, USA) also confirmed BRCA2 VUS. Conclusion: BRCA VUS identifies ovarian cancer patients- related molecular alterations from database consultant. Although the VUS are associated with ovarian neoplasms, their pathogenetic role is uncertain. However, the identification of this alteration strengthen the hypotesis of the neoplastic nature of the ovarian mass. E-PS-13-002 The national specialty exam in Spain; pathology questions related to images M.J. Gálvez*, F. Leiva-Cepas, I. Sánchez Ramírez, S. Haro Yuste, J. Osuna Soto, A. Sanz Zorrilla *Hospital Universitario Reina Sofía, Spain Background & objectives: Access to specialized medical training in Spain is carried out through a national and multidisciplinary test-type exam (MIR). The objective of this work is to analyse the questions directly related to Pathology specialty, evaluating their weight in the MIR exam. Methods: Questions from the exams officially published by the Minis- try of Health in the last 12 years (2010-2022) have been analysed. They have been grouped by year, according to whether they are associated with an image or not. Difficulty and discrimination indices were calcu- lated. They were analysed according to values of indices and grouped by year, blocks and types of question. Results: 16 questions with images (2015-2022) and 26 without images have been asked. The questions with images have dealt with nephropa- thology, chronic inflammation, digestive system and a miscellany. The questions without images are more varied and oscillate between molec- ular pathology and general pathology. Two questions were canceled in the period analysed. The questions in the exam are not grouped by blocks, and some of them are used as reserve questions. Conclusion: Pathology is a specialty whose representation in the MIR exam varies annually. There are no complete blocks of questions in Pathology. The introduction of images to the exam has kept the number of questions stable. E-PS-13-003 Revival of teaching anatomy, histology and pathology in Gdansk, Poland, after WWII J. Gulczynski*, A. Szarszewski, M. Michalski, E. Izycka-Swieszewska *Medical University of Gdansk, Poland Background & objectives: After WW II Doctors Academy was estab- lished in Gdansk in 1945. It used the premises of Medizinische Akad- emie in Danzig, created before the war by the Nazis. Majorority of new Professors came from University of Stefan Batory in Vilnius. Methods: We analysed available articles and papers presenting three most important scientists in anatomy, pathology and histology with embryology – professors Michał Reicher (1888-1973), Wilhelm Czar- nocki (1886-1963) and Stanisław Hiller (1891-1965), their work, teach- ing and research, but also how their students remembered them. Those students became later our teachers and passed the strive for develop- ment upon next generations. Results: Those Professors were invited to a new place, with short dark chapter of Nazi medicine, asked to create new medical academy with full scope of specialties to educate new generations of medicals. Hiller graduated from Jagiellonian University, with Rockefeller Foundation scholarship studied at Yale and Cornell Universities, to become the Head of Histology and Embryology Department in Vilnius. Reicher studied anthropology and medicine, after scholarship in Carnegie Institute returned to Warsaw University in 1915, became the Head of Anatomy Department, in 1920 nominated position in Vilnius. Czar- nocki graduated from Jagiellonian University, worked in Pathology Department at Warsaw University. Due to war outbreak never reached Vilnius, settled in Gdansk in 1945. Conclusion: Doctors Academy in Gdansk was founded in 1945, changed its name to Medical Academy in 1950. With ones of the best available teachers it quickly became one of the most important in Poland, releasing generations of new doctors and pharmacists. All of the mentioned above professors had strong impact on following gen- erations. Their manuals are in many aspects timeless and sometimes actual. Students should remember those researchers, not just the name- plates in our University lecture rooms they enter nowadays. E-PS-13-004 Forgotten war of the 20th century - Prof. Rudolf Weigl (1884-1957) and his fight against typhus J. Gulczynski*, M. Chodyna, M. Michalski, E. Izycka-Swieszewska *Medical University of Gdansk, Poland Background & objectives: Rudolf Weigl was Polish biologist and crea- tor of the first effective vaccine against typhus. The aim is to recall research and history of fighting this epidemic by creating method of obtaining a vaccine from lice, carriers of the disease-causing microorganism. Methods: We used biographical articles describing the life of Prof. Rudolf Weigl; literature and demographic and epidemiological data in the area of Poland from the period between the First and Second World War. We also analysed scientific articles by Rudolf Weigl describing his research and methods that resulted in obtaining the effective vaccine.