ECP 2023 Abstracts

S367 Virchows Archiv (2023) 483 (Suppl 1):S1–S391 13 E-PS-24 | E-Posters Uropathology E-PS-24-001 Does mean nucleolar volume have prognostic significance in seminomas? Y. Adali*, V. Şen, B. Tuna, O. Bozkurt, K. Yörükoğlu *Izmir University of Economics Faculty of Medicine, Turkey Background & objectives: Seminomas consists of large, tumour cells which contain one or two prominent nucleoli. In this study, it was aimed to examine the impact of the mean nucleolar volume (MnV) on progno- sis in seminomas, which has prognostic importance in various tumours. Methods: 54 cases diagnosed as pure seminoma were included in the study. The section of tumour was photographed at 400X magnifica- tion and digitally enlarged. The length of the nucleoli was measured using a transparent direction finder and ruler at a total magnification of 1200x. For each case,15 cells were counted and MnV was calculated stereologically. Clinical data were compared with MnV. Results: The mean tumour diameters were 59.2±31.4, and 33 (61.1%) of the cases were pT1, 8 (14.8%) pT2, and 13 (24.1%) pT3. The MnV of the cases was calculated as 2471.8±1072.3 μm³. MnV was found to be increased as the tumour diameter increased (p= 0.000). No statistically significant correlation was observed between the pT stages and MnV (p= 0.762). 9 (16.7%) cases showed biomarker recurrence, 4(7.4%) cases had metastasis and the mean disease-free survival was 6.4±4.1 years. The MnV of the cases with metastasis was 2712.3±1763.0 μm³, and without metastasis 2452.5±1023.8 μm³; revealing no statistical significance (p= 0.645). It was observed that higher MnV was related with better survival (p= 0.000). Conclusion: The present study revealed a relationship between MnV and tumour diameter. As MnV increases, the increase in survival seems to be inverse to the relationship with tumour diameter. However, in cases known to be manifested mostly with a painless mass, it can be thought that the increase in tumour size as MnV increases, leads to an association with a rise in the probability of early diagnosis and there- fore a better survival. E-PS-24-003 PTEN and ERG status and relationship in a prostate cancer cohort from Jordanian Arab population S. Al Bashir*, M. Alorjani, I. Matalka, M. Matalka, K. Kheirallah *Jordan University of Science and Technology, Jordan Background & objectives: ETS-related gene (ERG) gene rearrange- ments and phosphatase tensin homologue (PTEN) deletions are ones of the most common genetic aberrations occurring in prostate cancer (PCa). However, their relations and patterns of expression in PCa in Jordanian population are not well studied. Methods: We retrieved 108 PCa specimens from the archives of the King Abdullah University Hospital, spanning a period from 2005 to 2022. These cases included 48 biopsies, 37 prostatectomies (RP) and 33 (TURP). PTEN and ERG expression status were assessed by immunohistochemistry and correlated with other clinicopathologic parameters (patient age, preoperative PSA, Gleason Score (GS)/ Grade group(GG) , and biochemical recurrence. Results: PTEN loss of any degree was observed in 45.3 % of the pros- tate cancer cases. ERG was expressed in 85% of the cases. There was an increased loss of PTEN expression with increasing the severity of the PCa (GS6 (GG1) to GS9-10 (GG5)). PTEN loss prostate cancer cases were less likely to be ERG Negative (9%). 36% of the total samples were PTEN loss and ERG positive cases and this was not statically significant. Conclusion: In this first study to address the question of PTEN loss and ERG status in a predominantly Jordanian Arab population, we docu- mented that PTEN loss are associated with more aggressive prostate cancers with higher Gleason scores/ Grades. PTEN loss prostate cancer cases were less likely to be ERG Negative. These findings support a rationale of screening for these biomarkers for prognostic purposes and molecular subtyping of the disease. E-PS-24-004 Sporadic renal hemangioblastoma: a rare benign vascular renal tumour A. Al-Zakwani*, M. Al-Riyami *Oman medical speciality board, Oman Background & objectives: Hemangioblastoma is a benign vascular tumour that is typically arise in the cerebellum. Renal hemangioblas- toma cases are seldom seen and usually accompany VHL disease. It shows histological morphology features similar to its cerebellar counterpart. Methods: N/A Results: Background: Hemangioblastoma is a benign vascular tumour that is typically arise in the cerebellum. Renal hemangioblastoma cases are seldom seen and usually accompany VHL disease. It shows histological morphology features similar to its cerebellar counterpart and it is frequently misdiagnosed as various malignancies such as renal cell carcinoma (RCC), epithelioid angiomyolipoma and epithe- lioid hemangiopericytoma. Immunohistochemistry is crucial to dif- ferentiate it from the other malignant renal neoplasms. Objective: This case report describes one case of sporadic renal hemangioblastoma happened in a 72-year-old male, presenting with vague right flank abdominal pain. The radiological findings were suggestive of renal cell carcinoma. Conclusion: 0 E-PS-24-005 The prevalence of SPOP gene mutations in prostate cancer: a ret- rospective cohort study M. Alorjani*, S. Al Bashir, M. Al Zoubi *Jordan University of Science and Technology, Jordan Background & objectives: Speckle-type POZ (SPOP) gene mutations were reported in primary human prostate cancer (PCa). Isocitrate dehy- drogenase-1 (IDH1) oncogene mutation was found in only 1% of PCa. This study aimed to investigate prevalence of SPOP and IDH1 muta- tions in PCa in Jordan. Methods: One hundred formalin-fixed paraffin-embedded tissue sam- ples were collected from Jordanian patients diagnosed with prostate adenocarcinoma. The obtained specimens were subjected to genomic DNA extraction, PCR amplification and direct sequencing of exons 4, 5, 6 and 7 for the SPOP gene and exon 6 for the IDH1 gene. Results: The SPOP gene mutations were found in 17 % of PCa cases, while no mutation was detected in the screened exon 6 of the IDH1 gene. Conclusion: The current study confirmed the presence of a high fre- quency of SPOP gene mutations in prostate cancer in the Jordanian Arab population; reported in four exons in 17 % of the studied PCa patients, consistent with findings of previous reports from other popu- lations. Most of these mutations were found in exon 7 representing the hotspot exon in PCa cases. On the other hand, no tested sample showed the presence of IDH1 mutation in the screened exon 6 of the gene. Funding: This research was funded by the Deanship of Scientific Research and Graduate Studies at Yarmouk University (grant number 12/2020)