ECP 2023 Abstracts

S390 Virchows Archiv (2023) 483 (Suppl 1):S1–S391 13 E-PS-25-008 The opinion of pathology resident physicians on their training as specialists in Spain F. Leiva-Cepas*, A. Sanz Zorrilla, M.J. Galvez Medina, I. Sánchez Ramírez, S. Haro Yuste, J. Osuna Soto *University Hospital Reina Sofia, Spain Background & objectives: The objective of this study is to know the opinion of the residents about the role of their residence tutor, to analyse the characteristics more focused on teaching and to identify the needs that the residents demand from their tutors. Methods: A cross-sectional study was carried out through a question- naire addressed to residents in their first-fourth year of specialization. The survey consisted of 24 items and a "Google Form" was used for the survey, with several options for each Likert-type question. The survey was disseminated through different media and through the Spanish Society of Pathological Anatomy. Results: The survey was disseminated for 12 months, in the period of time prior to the incorporation of the new first-year residents (Sep- tember 2020). The total sample consisted of 186 answers per multiple choice question and 92 answers in the open question of free response. Of the 186 responses, 21 corresponded to first-year specialists and 12 to second-year specialists. 153 residents of the 4 years of training responded, 35.01% of the total (437 places offered in the last 4 years). This sample is considered representative with a correlation factor of 0.91 according to the regression line between the surveys received and the number of MIR by autonomous community. Conclusion: Among the different hospitals, there was heterogeneity in the training, the positive evaluation of the tutors and their temporary involvement. Resident physicians consider that little research and case studies are done during training. E-PS-25-009 Steroidogenic acute regulatory (StAR) protein is a useful marker for sex-cord-stroma tumours and normal/neoplastic adrenocortical tissue M. Lennartz*, D. Amirzada, N. Blessin, C. Fraune, N. Gorbokon, C. Hube-Magg, T. Krech, A. Hinsch, E. Burandt, G. Sauter, R. Simon, M. Kluth, F. Jacobsen, C. Bernreuther, S. Steurer *University Medical Center Hamburg-Eppendorf, Germany Background & objectives: Steroidogenic acute regulatory (StAR) protein is a mitochondrial transport protein with a critical regulatory role for steroid hormone production. As its expression is limited to few normal tissues, the immunohistochemical analysis of StAR was proposed to be diagnostically useful. Methods: To comprehensively evaluate the diagnostic utility of immu- nohistochemical StAR expression analysis, a tissue microarray con- taining 19,202 samples from 152 different tumour types and subtypes and 608 samples of 76 different normal tissue types was analysed by immunohistochemistry (IHC). Melan-A “cross-reactive” data were available from a previous study for comparison. Results: StAR immunostaining occurred in 198 (1.2%) of the 17,135 analyzable tumours. StAR expression was observed in 27 of 152 tumour categories, 9 of which included at least one strongly positive case. The highest rate of StAR positivity occurred in Leydig cell tumours of the testis and the ovary (100%), steroid cell tumours of the ovary (100%), adrenocortical carcinomas (93%) and adenomas (87%), Sertoli-Leydig cell tumours (67%) and granulosa cell tumours of the ovary (56%) as well as in seminomas (7%). As compared to Melan A, StAR was more often positive in adrenocortical neoplasms and in Leydig cell tumours while StAR (but not Melan-A) was negative in Sertoli cell tumours. Conclusion: Our data provide a complete overview on the patterns of StAR immunostaining in human tumours and suggest a diagnostic utility of StAR immunohistochemistry for supporting a diagnosis of Leydig cell tumours or of normal or neoplastic adrenocortical tissue. In contrast to Melan A, StAR appears to be more sensitive for these tumours while StAR is always negative in malignant melanoma, a fre- quent source of adrenal metastasis. E-PS-25-010 Dielectric permittivity analysis of human tissues S. Lopez-Prades*, M. Torrecilla, M. Rus-Villena, A. Muñoz, M.T. Rodrigo, I. Archilla, A. Diaz, J.M. O’Callaghan, M. Cuatrecasas *Hospital Clinic, Barcelona, Spain Background & objectives: Dielectric permittivity (DP) characterizes the interaction of tissues when exposed to an external electric field. Tissues with different cellular composition will provide different DP values. We aim to determine DP values in healthy human tissues to create a database. Methods: Prospective observational study of all types of healthy human tissues. Fresh tissue samples are placed in a Teflon® base placed on a scale to ensure optimal pressure (1g) of the coaxial probe for real time DP measurement. After DP measurement, we select the measured area, and conventional FFPE process is performed. H&E slides are reviewed and annotated for any changes. Results: We analysed 110 healthy tissues from 27 patients, obtained from surgical specimens or autopsies of less than 12 hours: 6 CNS, 1 thyroid, 13 lung, 12 spleen, 15 liver, 7 kidney, 13 salivary gland, 15 fat, 13 skeletal muscle, 14 heart, and 1 tongue. The semi-logarithmic DP graphs showed different patterns depending on the type of tissue. Fat DP values were the most characteristic, showing an almost linear curve, with DP values between 0 and 5, way below other tissue measures. Of notice, histologically visible intrahepatic and intramuscular fat could be predicted by DP measurements, which were lower than permittivity from other liver and muscle samples. Conclusion: There are differences in dielectric permittivity between different types of healthy human tissues. The DP analysis allows the identification of certain alterations, such as the presence of intrahepatic or intramuscular fat. A DP database of healthy tissues will provide the basis for future applications and could help in the discrimination between healthy and tumoral tissues, among other pathologies. E-PS-26 | E-Posters One-Day Molecular Diagnostics Pathology Symposium E-PS-26-001 Platelets–lymphocytes network biomarkers in haematological malignancies by flow cytometry methods E. Matei*, A. Chisoi, A. Mitroi, M. Aschie, M. Cristian, E. Gheo- rghe, N. Dobrin, M. Manea *1 Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology, “Ovidius” University of Constanţa, Romania Background & objectives: Acute myeloblastic leukaemia (AML) is characterized by the clonal proliferation of malignant hematopoietic precursor cells in the hematogenous marrow, and non-Hodgkin lym- phomas represent malignancies characterized by a monoclonal prolif- eration of B-cell (BCL) or rarely T-cell.