ECP 2017 - Abstract Book

exposure to ultraviolet (UV) radiation. Solar elastosis (SO) represents the

photodamage caused by UV radiation in lip lamina propria. This study

aimed to assess the extension and density of SO and its relation with

different degrees of epithelial dysplasia in AC.

Method:

SO was evaluated through histochemical reactions with

Picrossirius Red andWeigert

’

s risorcin-fuchsin. Epithelial dysplasia was eval-

uated according to WHO grading system. Elastosis extension was measured

and scored 1

–

4 according to elastic fibres density. Student

’

s t test and logis-

tical regression were performed and statistical significance was set at

< 0.05.

Results:

Elastotic material was positively stained with risorcin-fuchsin.

AC cases with moderate and severe epithelial dysplasia had an increased

extension of SO and higher scores of density. SO was closer to the epi-

thelium in cases with severe dysplasia (

< 0.05).

Conclusion:

We observed that SO is composed mainly by degenerated

elastic fibres and that it is significantly related to epithelial dysplasia

grading in cases of AC. Therefore, the extension and density of

photodamage in connective tissue reflect the epithelial alterations that

occur in AC.

PS-09-074

Oncocytic lipoadenoma of the submandibular gland: A peculiar case

of a rare entity

T. Buhagiar

, M. Ceci, A. Betts

Mater Dei Hospital, Histopathology, Msida, Malta

Objective:

Oncocytic lipoadenoma is an extremely rare salivary gland

tumour with less than 30 cases reported in the literature to date. These

tumours primarily occur in elderly males, usually in the parotid gland,

followed by the minor salivary glands than the submandibular gland.

Method:

We report a case of oncocytic lipoadenoma occurring in the

submandibular gland of a 50 year old female. A comprehensive literature

review was carried out and the findings tabulated and compared with our

case.

Results:

The patient presented with a submandibular mass seen on neck

ultrasound. FNA of this mass was non-diagnostic with only a few clusters

of benign acinar cells being present. A formal excision was done, and

macroscopic examination of the specimen showed a well circumscribed

tumour with a homogenous yellow cut surface. On microscopy the tu-

mour was unencapsulated, and was predominantly composed of sheets of

mature adipocytes in which aggregates of oncocytes as well as occasional

ducts and groups of acinar cells were seen.

Conclusion:

Oncocytic lipoadenoma is a rare benign tumour most com-

monly occurring in the parotid gland, with only a handful of cases occur-

ring in the submandibular gland reported in the literature. The differential

diagnosis includes sialolipoma and salivary gland oncocytic tumours.

PS-09-075

A large nasal mass revealing a primitive thyroid carcinoma

N. Mhamdi

, M. Belakthar, S. Mestiri, N. Abdessayed, M. Guerfala, S.

Chaieb, S. Chouchane, M. Abdelkafi, M. Mokni

Farhat Hached Hospital, Pathology Dept., Sousse, Tunisia

Objective:

Metastasis of thyroid carcinoma usually occurs in lymph

nodes or bones, however other sites such naso-sinusian mucosa are ex-

tremely rare. To our knowledge only three cases of nasosinian metastasis

of a thyroid carcinoma have been described in the literature. We report a

new Tunisian case.

Method:

A 43-year-old patient consulting for paresthesia of the right

hemi-face associated with nasal obstruction. Physical examination re-

vealed a mass of the nasal fossa with a large thyroid nodule. In imaging,

it was a large mass of the right spheno palatine fossa that extends from the

tonsillar sella to the right maxillary gland. A biopsy of the nasal lesion

was performed.

Results:

The biopsy revealed a poorly differentiated carcinoma (PDC),

arranged in nests and cordons. At immunochemistry, tumour cells

expressed CK and thyroglobulin.

Conclusion:

PDC of the thyroid is an aggressive malignant tumour. In

addition to local invasion, the majority of patients present at the time of

diagnosis distant metastases. A revealing nasal location is exceptional.

Since it is a poorly differentiated tumour, it poses a major diagnostic problem.

The primordial immunochemical study by a thyroid marker is necessary.

PS-09-076

Bone tumours of the face: Anatomo-clinical study of 26 cases

I. Msakni

, F. Gargouri, M. Mhiri, N. Mansouri, C. Kammoun, A.

Bouziani, B. Laabidi

Hôpital Militaire de tunis, ANAPATH, Tunisia

Objective:

Bone tumours of the face (BTF) gather very varied histolog-

ical entities. Radiological examination would often suggest the diagnosis

and histological examination is mandatory to confirm the diagnosis. The

aim of our work is to study the epidemiological and clinical profile of

BTF as well as their pathological characteristics and their evolution.

Method:

Our retrospective study included 26 BTFs, collected between

2000 and 2014.

Results:

The mean age was 36,9 years with a sex ratio of 2,2. Clinical

signs were dominated by swelling. Our series included 21 benign tumours

(81 %) and 5 malignant tumours (19 %). These were 12 odontogenic

tumours: 8 keratocysts, 1 dentigerous cyst, 1 ameloblastoma, 1 complex

odontoma and 1 odontogenic fibroma. The study showed 14 non-

odontogenic tumours: 5 chondrosarcomas, 4 osteomas, 1 fibrous dyspla-

sia, 1 osteoid osteoma, 1 ossifying fibroma, 1 osteochondroma, and

histiocytosis X. The treatment was surgical in all cases. Complete tumour

excision was performed in 18 patients. Radical surgery was performed in

8 patients with osteomas and chondrosarcomas. A patient with grade III

chondrosarcoma had radiotherapy and two patients with grade II

chondrosarcoma received chemotherapy. A tumour recurrence occurred

in 9 (35 %) patients with osteomas, chondrosarcoma, keratocyst, fibrous

dysplasia, and ameloblastoma.

Conclusion:

Because of their scarcity and diversity, BTFs present a di-

agnostic dilemma. The pathologist must rely on clinical data and mor-

phological findings to establish the adequate diagnosis.

PS-10-001

MicroRNA signature associated with KRAS-mutated colorectal cancer

O.-M. Andreoiu

, M. Dobre, I.-R. Pirvu, F. Vasilescu, V. Herlea, G.

Becheanu

Emergency University Hospital, Dept. of Pathology, Bucharest, Romania

Objective:

KRAS gene mutations play an important role in the

activation of RAS-RAF-MEK-ERK signaling pathway in colorec-

tal cancer (CRC) progresion. Recent studies have shown that

microRNAs (miRNAs) signature is associated with specific tu-

mour subtypes. This study aimed to determine the miRNA signa-

ture in KRAS-mutated CRC.

Method:

We studied fresh-frozen fixed in RNAlater tumour fragments

from 18 patients with CRC. To identify miRNA signature we used

Human Cancer Pathway Finder miRNA PCR Array, Qiagen, USA, com-

paring 10 mutant with 8 wild-type KRAS tumours (codons 12, 13, 59, 61,

117 and 146). Analysis of the results was performed using Free miRNA

PCR Array Data Analysis, Qiagen, USA.

Monday, 4 September 2017, 09:30

–

10:30, Hall 3

PS-10 Molecular Pathology

Virchows Arch

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2017

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