186 / 366
50 years (range, 18
–
80 years), with no gender predilection. The differen-
tial diagnosis of conjunctival myxoma includes amelanotic melanoma,
fibrous histocytoma, lymphangioma, amelanotic nevus, lymphoma,
myxoid neurofibroma, spindle cell lymphoma or rhabdomyosarcoma.
Conjunctival myxoma can be misdiagnosed as a conjunctival cyst.
Conjunctival myxomas can occur in association with the Carney
Complex, which is an autosomal dominant syndrome associated with
benign tumours, spotty mucocutaneous pigmentation, and endocrine
overactivity.
PS-11-011
Orbital cystic schwannoma arising from optic nerve
Z. Bayramoglu
*
, E. Ayik, C. I. Bassorgun, G. O. Elpek
*
Akdeniz University, School of Medicine, Pathology, Antalya, Turkey
Objective:
Most of the primary tumours of optic nerve are meningiomas
and gliomas. Schwannomas arising from optic nerve are extremely rare
lesions and only a few case has been reported until today. These lesions
are slow-growing, well-circumscribed, solid/cystic masses. Here we re-
ported a rare lesion schwannoma located in optic nerve with a cystic
morphology.
Method:
case report
Results:
A 57-year-old female patient presented with a left ocular pain
and progressive left proptosis. The patient was otherwise healthy. Orbital
magnetic resonance imaging revealed an intraconal heterogeneous mass
of 25 mm × 18 mm, radiographically consistent with a cavernous hem-
angioma. Surgical resection was performed and according to histopatho-
logical and immunohistochemical examination the final diagnosis was
determined as
“
Orbital Schwannoma with Cystic Morphology
”
.
Conclusion:
Peripheral nerve tumours comprises 2 % of all orbital tu-
mours and schwannomas are the most common types. Schwannomas are
benign tumours arising from Schwann cells and most commonly involve
trigeminal nerve root in intracranial location. Even if it is rare,
schwannomas arising from optic nerve can be seen and cystic morphol-
ogy of these tumours should not mislead the diagnosis.
PS-11-012
Keratoplasty in patients with Acanthamoeba keratitis: A study of
three cases
L. Alfaro
*
, C. Peris-Martinez, M. Roca-Estelles
*
Valencia, Spain
Objective:
Corneal infections by amoebas present difficulties for clinical
diagnosis and may be confused with other keratitis of the herpetic, fungal
or bacterial type. We reviewed the clinical features and evolution in three
patients with amoebic keratitis who required corneal transplantation after
failure of medical treatment.
Method:
The studied samples were three specimens of penetrating kera-
toplasty. Patients were two women aged 22 and 28 years and a 40- year-
old male. One patient underwent a small corneal biopsy 1 week before
transplantation, which was negative for amoebae. Samples were proc-
essed in a conventional manner and in addition to H/E sections
Trichrome, PAS and Gram techniques were performed.
Results:
The three patients revealed corneal cysts and trophozoites of
Acanthamoeba. The epithelium was detached in all three although only
two presented significant ulceration. One of the patients who had devel-
oped a crystalline keratopathy had superficial stromal band deposits
interpreted by accumulation of the drugs used in their treatment.
Conclusion:
The difficulty of reaching a clinical diagnosis of certain-
ty is reflected in the negativity of cultures in all three patients al-
though all had a history of contact lens use. For this reason they
had received combined antibacterial and ant-amoebic treatment and
one of them antifungal.
PS-11-013
New diagnostic approaches to uveal melanoma: EGFR, TGFb and
MMP9 expression correlate with histological type and invasiveness
N. Danilova
*
, S. Davidova, P. Malkov
*
Lomonosov Moscow State University, Dept. of Pathology, Russia
Objective:
The purpose of the present studywas to investigate the relationship
betweenMMP9 expression and scleral invasion of uveal melanoma (UM). We
also examined the effect of growth factors (TGFb and EGF), oncosuppressor
protein (p16) on the histological types and mitotic activity of tumour.
Method:
Tumour specimens were obtained from 42 primary UM imme-
diately after enucleation.
Results:
Hyperexpression of MMP9 and EGFR were correlated with a high
proportion of spindle cells in UM (Kruskal-Wallis test
p
< 0,05 for each).
Moreover, we have demonstrated the association between the level of EGFR,
TGFb andMMP9 expression to the initial stage of tumour invasion (Spearman
’
s
test
p
< 0,05). Moreover, there was a correlation between TGFb hyper-
expression and mitotic activity (Spearman
’
s test
p
= 0,059). Furthermore, a
low level of p16 expression in UMwas proportional hyperexpression of TGFb.
Conclusion:
EGFR and MMP9 are known to be used as targets for anti-
cancer therapy. The results of our study are suggesting to develop newer
approaches of UM treatment on the early stages of invasion in order to keep
an affected eye as an organ. Thereupon, it was concluded about the key-role
of abnormalities in TGFb-pathway that cause the down-regulation of p16-
gene, where the latter may lead to increased mitotic rate.
PS-12-001
Skeletal muscle biopsy in the diagnostic algorithm of rare cardiomy-
opathies - a retrospective study
M.-C. Dinca
*
, D.-A. Costache, P.-B. Harsan, C. G. Socoliuc, E. Gramada,
E. Manole, A. Bastian
*
Colentina University Clinical Hospital, Dept. of Pathology, Bucharest,
Romania
Objective:
To highlight the usefulness of performing skeletal muscle
biopsies in the work-up of cardiomyopathies with skeletal muscle in-
volvement. Cardiac abnormalities are common during the evolution of
many neuromuscular diseases, manifesting as dilated or hypertrophic
cardiomyopathies, arrhythmias or conduction disturbances. While in
most cases the onset is late, the cardiac symptoms may dominate the
clinical picture or even precede the myopathic signs.
Method:
We retrospectively reviewed 463 consecutive muscle biopsies
performed and analyzed in a 3 years period (2014
–
2016), using the
Colentina Clinical Hospital Pathology Department database.
Results:
The skeletal muscle tissue was obtained using open biopsy un-
der local anesthesia; muscle cryosections served for histological,
histoenzimological, immunohistochemical stains and complementary
techniques of western blotting and electron microscopy. We diagnosed
two cases of desmin-related myofibrillar myopathies, one later proved to
be caused by a newly identified form of mutation in desmin, the other
carrying an additional mutation in alpha-B crystalline gene, two cases of
acid maltase deficiency with cardiac involvement, one case of Danon
disease, a multi-minicore myopathy with cardiomyopathy and one gam-
ma sarcoglycanopathy with severe cardiac signs.
Conclusion:
The morphological aspects enabled us to establish the diag-
nosis with major impact on clinical management and directed further
confirmatory genetic testing.
Tuesday, 5 September 2017, 09:30
–
10:30, Hall 3
PS-12 Cardiovascular Pathology
Virchows Arch
(
2017
)
471
(
Suppl 1
):
S1
–
S352
S172