83 / 366
Conclusion:
In conclusion, our results indicate that breast cancer in pa-
tients
≥
90 years old exhibits larger size, high grade and prevalence of
invasive ductal carcinoma. Molecular classification reveals a predomi-
nance of luminal B tumours in these patients.
PS-02-002
Immunohistochemical study of 500 cases of neuroendocrine tumours
D. Alshaar
*
, D. ALSHAAR
*
Al Assad University Hospital, Damascus, Syrian Arab Republic
Objective:
Our study is an approach of grade and stage of neuroendo-
crine tumours diagnosed between Jan 2006 and Dec 2016 at Al-Assad
university hospital/Damascus University, and to apply newly WHO ap-
proved classifications of these tumours depending on site of origin.
Method:
We studied H&E stained sections, and multiple immunostains
(Synaptophysin, Chromogranin, CD56, and CKs) to confirm the type,
mitotic activity was evaluated by light microscopy and Ki-67.
Results:
The patients age range between 18 and 72 year old, male to female
ratio 312:188. The first three sites of higher incidences of primary neoplasias
were Lung with 211 case, then GI tract and Pancreas with 131 case, the
adrenal glands with 70 case, the remaining cases were presented in the
thyroid, hepatic metastases, brain metastases and abdominal masses.
Synaptophysin showed positive reaction on 90 % of studied
cases,Chromogranin on 78 % and CD56 on about 54 % . Ki-67 evaluation
felt into three groups : less than 3 % for well differentiated low grade tumours
( 204 case), 3 to 20 % for intermediate grades (121 case), and more than 20%
of high grade (175 case),high-grade nearly always presents with high-stage.
Conclusion:
Neuroendocrine tumours are unique tumours that need
to be confirmed by available methods and techniques, and given a
meticulous evaluation of grade and stage, because of what that
may affect the prognosis of the patient case and method of treat-
ment.
PS-02-003
Papillary thyroid microcarcinoma: Retrospective study
P. Rodrigues
*
, F. Costa, M. J. Martins
*
CHUC, Anatomia Patológica, Coimbra, Portugal
Objective:
Papillary thyroid microcarcinoma (PTMC) is defined after its size
till 10 mm or less. PTMC diagnosis increased in pathology routine concerning
almost 50 % of all papillary carcinomas. In near future, accurate diagnosis
correlated with histopathological variants and prognosis parameters.
Method:
A series of 369 PTMC was reviewed as a retrospective 10 years
study in Centro Hospitalar e Universitário de Coimbra, collected from
thyroid carcinomas surgical specimen and reclassified according to the
8th AJCC of the 2017. Histophatological type, patient gender/age,
bilaterality and mutifocality were the searched parameters.
Results:
Female gender prevalence (87,3 %) was consist as already
known and 56 years (between 21 and 80 years old) was the medium
age. Multifocal tumours were found in 25,7 % (19,6 % bifocal, 5,1 %
trifocal and 1 % with more than three tumours) of cases.
Histopathologically we found non encapsulated follicular PTMC, non
encapsulated classical type, encapsulated classical and encapsulated fol-
licular variants in 46.6 %, 27.4 %, 11.9 and 11.1 % respectively. All cases
of the PTMC were now reclassified as pT1a.
Conclusion:
These results are in accordance with international casuistic.
The increasing incidence of PTMC is actually due to the improvement of
the clinical diagnostic acuity, together with the increase of surgical pro-
cedures and pathology concern.
PS-02-004
Differential diagnosis between benign and malignant thyroid nodules
using Second Harmonic Generation Microscopy
L.-G. Eftimie
*
, R. Hristu, O. C. Voinea, M. Dumitrescu, G. Stanciu, M.
Sajin
*
Central Military Hospital, Pathology, Bucharest, Romania
Objective:
The purpose of this study was to compare the ultrastructural
capsular changes appearing in follicular adenoma, papillary and follicular
thyroid carcinoma for differentiating between benign and malignant thy-
roid nodules.
Method:
Second Harmonic Generation(SHG) Microscopy was used to
image collagen distribution in the capsules of several types of nodules.
The tissue fragments were formalin-fixed, paraffin-embedded but without
H&E staining, with 4
–
7 microns thick sections. Collagen organization
was evaluated using different parameters such as the collagen organiza-
tion coefficient based on the Fast Fourier Transform(FFT) of 2D-images
and the Gray-Level Co-Occurrence Matrix(GLCM) angular second
moment(ASM) used as a measure of textural uniformity and entropy
which is a parameter of disorder in a micrograph.
Results:
SHG microscopy images were acquired to assess the collagen
organization of tumoural capsular thyroid nodules previously diagnosed
as benign or malignant by conventional H&E staining. Different degree
of collagen fibers organization was observed and quantified using FFT
and GLCM. A higher degree of structural variation was observed for the
thyroid capsule compared to nodular capsules resulting in lower value of
ASM and higher entropy.
Conclusion:
These above described microscopy method help us distingues
between benign or malignant thyroid nodules, based on the capsular colagen
parameters, especially in patients treated with antithyroid drugs.
PS-02-006
Immunohistochemical expression of CD1a in different tumours of
pituitary gland
S. Simonetti
*
, E. Musulen, C. Carrato, A. Hernández, I. Koptseva, M. A.
Carrasco
*
General Hospital of Catalonia, Dept. of Pathology, Barcelona, Spain
Objective:
Recently it has described that expression of CD1a could be
useful to distinguish adenomas from non-neoplastic pituitary parenchy-
ma, with positive staining in normal parenchyma and negative staining in
adenomas. In our study we analyzed the immunohistochemical expres-
sion of CD1a in tumoural and normal pituitary samples with the aim of
verifying the utility of this staining in different lesions of hypophysis.
Method:
Immunohistochemical staining was performed using the auto-
mated staining device Ventana BenchMark Ultra (Roche Diagnostics,
Mannheim, Germany) and the antibody was the rabbit antibody against
CD1a (clone EP3622, Roche Diagnostics).
Results:
A total of 24 samples (13 adenomas, 8 normal hypophysis, 2
atypical macroadenoma and 1 craniopharyngioma) were analyzed. Three
adenomas were completely negative for CD1a staining and 10 cases
showed scattered positive cells. All cases of normal tissue showed posi-
tivity for CD1a. Moreover we found that the 2 atypical macroadenomas
showed intense CD1a immunoreactivity and the craniopharyngioma sam-
ple showed isolated positive cells.
Conclusion:
In our study we found that CD1a is not a very useful marker for
distinguishing adenomas from normal tissue hypophysis, because we found
scattered positive cells the majority of adenomas and the craniopharyngioma
and diffuse positivity in two cases of atypical macroadenomas.
PS-02-007
The usefulness of parathyroid hormone measurement in fine needle
aspiration biopsy washout fluids of parathyroid neoplasm
Sunday, 3 September 2017, 09:30
–
10:30, Hall 3
PS-02 Endocrine Pathology
Virchows Arch
(
2017
)
471
(
Suppl 1
):
S1
–
S352
S69