116 / 366
normal microbiome pattern revealed mild chronic inflammation without any
active lesion. On immunohistochemical staining, CCC with dybiosis showed
focal p16 expression, weak membranous EGFR and HER2 expression, and
higher Ki-67 labelling. However, CCC without dysbiosis showed negative
expressions of p16, EGFR and HER2 with low Ki-67 labelling. No KRAS
mutation was identified in both groups.
Conclusion:
We identified normal flora in bile juice of healthy individ-
uals and demonstrated association of dysbiosis with GB diseases. The
histologic findings and immunohistochemical results also can support
that dysbiosis may be associated with chronic active lesion with epithelial
atypia, which can lead to develop precancerous or malignant lesion.
PS-05-001
Is peritumoural lymphocyte infiltration predictor for PDL-1 positive
patients with locally advanced or metastatic non-small cell lung can-
cer (NSCLC)?
Z. Yildirim Ekin
*
, D. Nart, U. Aykutlu, T. Göksel, A. Veral
*
Ege University Faculty of Medicine, Dept. of Pathology, Izmir, Turkey
Objective:
Tumour programmed death ligand one (PD-L1) which is the
ligand for programmed death receptor-1 (PD-1); the expression of PD-L1
on a target cell binding to PD-1 can inhibit T-cell receptor signaling and
diminish interactions with dendritic cells, resulting in anergy. Immune
checkpoint inhibition has shifted treatment paradigms in non-small cell
lung cancer (NSCLC). T regulatory cells play important roles in immune
suppression, the reversal of which is vitally important for the success of
immune therapy. Conflicting results have been reported regarding the
immune infiltrate and programmed death-ligand 1 (PD-L1) as a prognos-
tic marker. We correlated the peritumoural immune infiltrate and PD-L1
expression with clinicopathologic characteristics of resected with locally
advanced or metastatic NSCLC
Method:
Tumour slides of surgical specimens of 50 cases with lung
cancer diagnosed in our department from 2012 to 2016 were analyzed
retrospectively. Immunohistochemistry was performed for PD-L1. Strong
PD-L1 expression was defined as greater than 50% tumour cell positivity.
Results:
The mean age was 59,6 years. 12 were female and 38 were males.
Of 50 patients, 15 were node-negative (N0), 2 N1 and 23 N2; 10 N3 34 were
adenocarcinomas (AC), 11 squamous cell cancers (SCC) and 5 other. Strong
PD-L1 expression was found in 18 % cases and weak PD-L1 expression was
found in 28 % cases. Strong peritumoural lymphocytes infiltration was found
in 24 % cases. PD-L1 expression was not associated with peritumoural lym-
phocyte statistically.
Conclusion:
PD-L1 expression and peritumoural lymphocytes were not
concordant in our study.
PS-05-002
Investigations on hematoxylin & eosin stained sections of acinar pul-
monary adenocarcinoma by confocal laser scanning microscopy
I. Dumitru
*
, S. G. Stanciu
*
Emergency University Hospital, Dept. of Anatomic Pathology,
Bucharest, Romania
Objective:
Lung cancer is the most common form of cancer in the world.
Imaging techniques such as Confocal Laser Scanning Microscopy
(CLSM) or Multiphoton Laser Scanning Microsopy (MLSM) can allevi-
ate part of the problems associated to conventional imaging techniques,
and in certain cases can eliminate the need for tissue removal. We attempt
to contribute in this regard by discussing a series of data sets collected on
H&E-stained sections of Acinar Pulmonary Adenocarcinoma by Bright
Microscopy (BM) and CLSM.
Method:
We employed a correlative imaging approach in which H&E
stained lung tissue sections were imaged with BM and CLSM. BM im-
ages have been collected using a Leica DM3000 system, while Confocal
Laser Scanning Microscopy data sets were acquired using a Nikon C2+
system working in fluorescence. For excitation a 488 nm laser beam was
used.The tissue was formalin-fixed and paraffin-embeded.
Results:
A series of analogies were established between the BM and
CLSM images. In CLSM images the neoplastic proliferation is delineated
by distinct borders consisting in fibrous bright septae. At closer magnifi-
cation, the acinar adenocarcinoma with gland formation and the nuclear
contours are well defined.
Conclusion:
CLSM and MPM provide the possibility to non-invasively
acquire in-focus images from selected depths from living, fixed and fro-
zen specimens.
PS-05-003
Comparison of PD-L1 mRNA expression measured with the
CheckPointTyper® assay with PD-L1 protein expression assessed
with immunohistochemistry (IHC) in lung cancer (NSCLC)
R. Erber
*
, R. Stöhr, S. Herlein, F. Fuchs, E. Veltrup, R. Wirtz, A.
Hartmann
*
University Clinics Erlangen, Dept. of Pathology, Germany
Objective:
PD-L1 evaluation in NSCLC became important since devel-
opment of anti-PD-L1/PD-1 drugs. Comparison of trials investigating
prediction of response to these drugs is complicated due to different
PD-L1 antibodies and cut-offs used. We analyzed comparability of PD-
L1 mRNA and protein expression and dependency on the way of tissue
acquisition.
Method:
Prospectively,
n
= 22 NSCLC cases (
n
= 9 EBUS TBNA,
n
= 5
metastases) were evaluated for PD-L1 protein on tumour cells (TC) and
immune cells (E1L3N, Cell Signalling; 28-8, Abcam) and PD-L1 mRNA
(CheckPoint TYPER® assay, STRATIFYER).
Results:
In primary NSCLC (
n
= 17), PD-L1 mRNA and 28-8 TC IHC
agreement was excellent (Kappa value = 0.85, p-value = 0.0002). In
EBUS-TBNA (
n
= 8), PD-L1 mRNA expression showed perfect agree-
ment with 28-8 antibody (Kappa value = 1.0, p-value = 0.0023). In me-
tastases, differences between PD-L1 mRNA and protein became apparent
(Kappa 0.2; p-value 0.2525). However, PD-L1 mRNA significantly dif-
fered when comparing 28-8 TC staining of tumours >49 % with 1
–
49 %
and 0 % (
p
= 0.0040;
p
= 0.0081, respectively).
Conclusion:
PD-L1 mRNA (CheckPoint TYPER®) and 28-8 protein
staining showed excellent agreement in primary NSCLC including lymph
node biopsies. Metastatic lesions require cut-off adoption according to
tissue type. PD-L1 protein expression in >50 % tumour cells by 28-8
antibody can be reliably detected by RT-qPCR from non-
macrodissected primary NSCLC tumour samples.
PS-05-004
Adequacy, clinicopathological and service parameters of molecular
testing on respiratory cytology specimens at a UK Tertiary Referral
Centre: A review of 64 cases from 2013 to 2016
Y. Z. Zhang
*
, R. Dina, C. Wright, H. E. Foong, P. Du Parcq, P. Viola, S.
Desai, N. Gupta
*
Imperial College Healthcare, Histopathology, London, United Kingdom
Objective:
Next generation sequencing (NGS) and ALK FISH are be-
coming routine practice for respiratory cytology specimens in identifying
actionable driver mutations, which is crucial in the management of met-
astatic lung cancer. We aim to assess our local specimen adequacy rates
and turnaround time from 2013 to 2016.
Sunday, 3 September 2017, 09:30
–
10:30, Hall 3
PS-05 Pulmonary Pathology
Virchows Arch
(
2017
)
471
(
Suppl 1
):
S1
–
S352
S102