ECP 2017 - Abstract Book

Method:

Baseline and on-treatment (Day 29) biopsies (

= 54) were

stained for CD8, PD-1, PD-L1, CD68, FoxP3 and CD4 by IHC.

Digitized slides were subjected to image analysis (tIA, CellMap 0.8 soft-

ware). Marker levels were compared using medians across the whole

group, by ipilimumab subgroups and by BOR subgroups [responders

(CR/PR), stable disease (SD), progressive disease (PD)]. Responses were

assessed with RECISTv1.1.

Results:

An increase was observed in all markers for the whole cohort,

primarily driven by increases in Ipi-N; however, substantial increases

were observed in CD8, PD-1 and PD-L1 in IPI-T responders. In the

IPI-N subgroup, CD8, PD-1 and PD-L1 increased in PD and SD but

did not change in responders. Both IPI-T and IPI-N subgroups showed

higher baseline CD8, PD-1 and PD-L1 in responders vs SD and PD.

Conclusion:

Patterns of immunomodulatory effect of nivolumab differ

by ipilimumab pre-treatment. Further work is required to determine the

significance of these differences and whether they underscore unique

mechanisms of anti-tumour response.

OFP-01-005

Vascularised composite tissue allograft pathology: Akdeniz

University experience

C. I. Bassorgun

, B. Unal, O. Dogan Ozkan, G. O. Elpek, O. Ozkan, M.

A. Ciftcioglu

Akdeniz University, Pathology, Antalya, Turkey

Objective:

Vascularized tissue allotransplantation differs from other solid

tissue transplantations in that it involves several different tissues.

Vascularized tissue allotransplantation pathology is important in detecting

changes in the event of rejection. The histopathological examination for

the detection of the rejection of vascularized tissue allotransplantation is

generally based on the Banff 2007 classification.

Method:

Routine skin biopsy specimens taken during the follow-up of

seven patients were evaluated for rejection. Findings were rated according

to the Banff 2007 classification. In a total of 96 biopsy specimens, find-

ings of grade I mild rejection were observed intensively, while grade II

moderate rejection was detected at secondary frequency.

Results:

Besides the histopathological findings in the Banff 2007 classi-

fication, some additional histopathological findings were detected in our

cases. The overlapping lesions were accompanied by drug eruption find-

ings of rejection. In addition, although the cases showed high rates of

grade I mild rejection findings contained in the Banff 2007 classification,

no clinical evidence of acute rejection was detected.

Conclusion:

We would like to present the findings that we observed

during the routine histopathological examination of the five face trans-

plant cases and two leg transplant cases performed by the Akdeniz

University Plastic and Reconstructive Surgery Department.

OFP-01-006

The assessment of clinical and histopathologic effects of PUVA and

NB-UVB in early stage mycosis fungoides

E. Yilmaz

Osmangazi University, Pathology, Eski

ehir, Turkey

Objective:

Mycosis Fungoides (MF), the most common form of T-cell

lymphoma, is staged as patchy, plaque and tumour forms. Although

PUVA and narrow-band UVB (NB-UVB) are the two most commonly

used treatment modalities in the early stages of disease, studies comparing

their histopathologic effects are scarce. The aim of our study is to com-

pare the clinical and histopathologic effects of PUVA and NB-UVB in

early stage MF.

Method:

The study included in 41 early stage MF cases treated with

either PUVA or NB-UVB. Both clinical and histopathologic responses

including the persistence of epidermotropism, changes in stratum

corneum and epidermis, dermal infiltrates, dermal fibrosis and other der-

mal and vascular changes were evaluated. Complications during treat-

ments were also noted.

Results:

Complete clinical responses were seen in 14 of 23 patients

(60.9 %) in the PUVA group and 11 of 18 patients (61.1 %) in the NB-

UVB group. The two groups showed significant differences in terms of

resolution of epidermotrophism, decrease in dermal infiltrates, and other

dermal and vascular changes.

Conclusion:

PUVA and NB-UVB have similar clinical and histopatho-

logic effects in the treatment of early stage MF.

OFP-01-007

Tertiary lymphoid structures in ameloblastoma

C. H. Siar

, Z. A. Bin Abdul Rahman, H. Tsujigiwa, H. Nagatsuka, K. H. Ng

University of Malaya, Oral & Maxillofacial Clin. Sciences, Kuala

Lumpur, Malaysia

Objective:

The ameloblastoma is a benign but locally-invasive

odontogenic epithelial tumour with a high recurrence rate after treatment.

Tertiary lymphoid structures (TLS) are ectopic lymphoid formations

representing an adaptive immune response to either specific pathogen,

inflammatory challenge or neoplastic process. Although these structures

are acknowledged measures of disease outcome in many cancer types,

their role in ameloblastoma remains unclear. To address this, we investi-

gated for their distribution, morphologic and immunophenotypic charac-

teristics, and evaluated their relevance.

Method:

Formalin-fixed paraffin-embedded specimens from 63 primary

and 14 recurrent ameloblastoma cases were subjected to immunohisto-

chemistry for expression of CD20, CD45RO, CD3, cortactin, NWASP,

WIP, RANK, RANKL and osteoprotegerin. Intra- and peri-tumoural lym-

phocytic infiltrate, lymphoid aggregates and TLS findings were correlat-

ed with clinicopathologic parameters.

Results:

There is a positive association between lymphocytic response

with tumour status (primary versus recurrent). Peritumoural lymphocytic

infiltrate, lymphoid aggregates and TLS were significantly higher in pa-

tients presenting with recurrent ameloblastoma (

> 0.05). Actin cyto-

skeletal regulators NWASP and WIP (except cortactin) overexpression

within TLS and lymphoid aggregates suggests enhanced motility of Tand

B lymphocytes. A low RANK-RANKL and high osteoprotegerin profile

within these lymphoid structures indicate an altered tumoural

osteoimmunologic microenvironment.

Conclusion:

Our results show that neogenesis of lymphoid organs do

occur in ameloblastoma albeit in low frequency. Their enhanced presence

in recurrent tumours may represent locally generated immune response

with potential antitumour activity to control growth and progression.

(Grant: FP032-2015A)

OFP-01-008

Significances of androgen receptor (AR), Her-2, S-100P,

Mammaglobin (MMG), AMACR expression in salivary pleomorphic

adenoma (PA): Its relationship to the malignant potential in PA

K. Kusafuka

, T. Kawasaki, T. Nakajima, T. Sugino

Shizuoka Cancer Center, Dept. of Pathology, Nagaizuimi, Japan

Objective:

PA is the most common benign tumour of the salivary glands.

Malignant change of PA is called

“

carcinoma ex pleomorphic adenoma

(CXPA)

”

, and its carcinomatous component frequently shows salivary duct

carcinoma (SDC). We aimed to elucidate the malignant potential in PA.

Method:

We selected PA (30 cases), atypical PA (APA: 5 cases), and

CXPA (20 cases). We examined AR, GCDFP-15, Her-2, MMG,

S-100P and AMACR expression, immunohistochemically.

Results:

The inner ductal cells in PA were focally positive for AR,

GCDFP-15, and S-100P, whereas they were very weakly and focally

Virchows Arch

(

2017

)

471

(

Suppl 1

–

S352