17 / 366
positive or negative for Her-2. In this study, the carcinomatous compo-
nent of CXPAwas composed of SDC, most of which were strongly and/or
diffusely positive for AR, GCDFP-15, Her-2, S-100P, MMG and
AMACR. The inner cells of APA histologically had the large eosinophilic
cytoplasm and nuclei with moderate atypia, and they were also positive
for such markers.
Conclusion:
According to AR and GCDFP-15 expression pattern, some
inner cells of PA and APA have biologically malignant potential with
apocrine differentiation, resembling the phenotype of SDC. The critical
point of malignant change is the overexpression of Her-2 and/or S-100P,
inducing aberrant MMG or AMACR expression.
OFP-01-009
Nasopharyngeal Carcinoma (NPC): Is there value in supplemental
testing for EBV, p16 or HPV?
M. Hyrcza
*
, T. Thomson, C. Poh, J. Laskin, J. Siever, H. Yau, A. Jagdis,
D. Hao
*
McMaster University, Dept. of Pathology and Molecular Medicine,
Hamilton, Canada
Objective:
Although NPC is associated with EBV, some cases instead
show evidence of HPV. It is not clear if determination of EBV and HPV
status in NPC is of clinical importance and should be routinely performed.
We examined 143 NPC cases to determine the prevalence of EBV and
HPV and their prognostic significance.
Method:
Tissue microarrays were constructed and the cores were tested
for EBV early RNA (EBER), p16 IHC and HPV RNA using in situ
probes for high-risk HPV.
Results:
Of the 143 cases , 133 were WHO Type III NPCs, 6 Type II, 1
Type I and 3 cores were missing. EBER was positive in 134 and p16 in 7
cases. Both were both positive in 5 cases. Among the 7 EBER- cases, 3
were p16+ and 4 negative. Three cases were positive for HPV RNA. The
5 year overall proportion surviving was 86 % (95 % CI = 79 %
–
90 %)
with a median time at risk of 52 months (range 3 to 120 months). Disease-
free survival at 5 years was 51 % (95 % CI = 42 %
–
59 %) with a median
time at risk of 37 months (range 3 to 120 months). Combined EBER-/
p16-negative cases had worse 2- and 5-year overall survival (p values
0.014 and 0.017 respectively). There were too fewHPV-positive cases for
outcome analysis.
Conclusion:
In this cohort, HPV+ cases were rare (3 %) and not predicted
by p16 testing. However, EBER-/p16- tumours had a worse prognosis,
suggesting routine testing of EBER-negative cases for p16 may have
prognostic utility.
OFP-01-010
Human papillomavirus-related carcinoma with adenoid cystic-like
features: A series of 5 cases expanding the pathologic spectrum
J.-F. Hang
*
, M.-S. Hsieh, C.-C. Pan, Y.-J. Kuo
*
Taipei Veterans General Hospital, Dept. of Pathology, Taiwan
Objective:
Human papillomavirus (HPV)-related carcinoma with ade-
noid cystic-like features is a newly described entity of the sinonasal tract.
Method:
We evaluated histomorphology, immunophenotype, and molec-
ular testing of 5 HPV-related carcinomas with adenoid cystic-like features
to identify potentially helpful features in distinguishing it from the clas-
sical adenoid cystic carcinoma (AdCC,
n
= 14) of sinonasal tract.
Results:
Comparing to AdCC, HPV-related carcinomas with adenoid
cystic-like features were associated with squamous dysplasia of surface
epithelium (80 % vs 0 %,
P
< 0.01) and presence of solid growth pattern
(100 % vs 29 %,
P
= 0.01), but less densely hyalinized tumour stroma
(20 % vs 86 %,
P
= 0.02). Squamous differentiation in the invasive
tumour was seen in 3 HPV-related carcinomas with adenoid cystic-like
features, two of them showing abrupt keratinization and 1 with scattered
squamous morules. Diffuse p16 staining in >50 % of tumour cells was
noted in all HPV-related carcinomas with adenoid cystic-like features but
only in 1 AdCC (100 % vs 7 %,
P
< 0.01). High-risk HPV testing was
positive in all HPV-related carcinomas with adenoid cystic-like features
(4 associated with type 33 and 1 type 16) but not AdCCs. MYB rear-
rangement was tested in 4 HPV-related carcinomas with adenoid cystic-
like features and all showed negative.
Conclusion:
We described novel pathologic findings of HPV-related car-
cinomas with adenoid cystic-like features, including squamous differen-
tiation and association with HPV type 16. Diffuse p16 staining followed
by HPV molecular testing is useful in distinguishing HPV-related carci-
nomas with adenoid cystic features from classical AdCCs.
OFP-01-011
Middle ear adenomatous neuroendocrine tumours: A 25-year expe-
rience at MD Anderson Cancer Center
D. Bell
*
, A. El-Naggar, P. Gidley
*
MD Anderson Cancer Center, Dept. of Pathology, Houston, USA
Objective:
Neuroendocrine tumours are uncommon in the head and neck
region and extremely rare in the middle ear. Therefore, the clinical and
pathologic characteristics of these tumours are less defined than neuroen-
docrine tumours of other sites. We reviewed our institutional experience
with middle ear adenomatous neuroendocrine tumours (MEANTs).
Method:
We searched our institution
’
s pathology files to identify patients
treated between 1990 and 2015 who had lesions classified as middle ear
adenomas, adenomatous tumours, adenomatous tumours with neuroendo-
crine differentiation, carcinoid tumours of the middle ear, low-grade neuro-
endocrine tumours of the middle ear, and neuroendocrine carcinomas of the
middle ear. When available, slides for the identified cases were retrieved
and re-reviewed by two experienced head and neck pathologists (DB,
AEN) to verify the histological diagnosis and exclude alternative diagnoses.
Results:
We identified 14 patients (9 women and 5 men) age 29
–
65 years
who received treatment for middle ear tumours at MD Anderson between
1990 and 2015. Although pathology slides were available for an additional
22 patients, no clinical follow-up data were available for these patients.
Conclusion:
Our report adds to the series cases of MEANTs with recur-
rences, lymph node involvement, distant metastases, and tumour-related
deaths. Our experience suggests that, although these tumours have long
been considered to be low-aggression neoplasms, long-term follow-up
studies to ascertain this supposed benignity are warranted. In conclusion,
our institutional experience with MEANTs demonstrates that these tu-
mours can recur, metastasize to the lymph nodes and distant sites, and
cause death.
OFP-01-012
Clinicopathological study of ameloblastoma: An experiential status
H. Salam
*
, T. Mirza, M. Irshad
*
Dow University of Health Sciences, Dept. of Oral Pathology, Karachi,
Pakistan
Objective:
With this experiential data, we aimed to identify the prevalent
pattern for presentation of ameloblastoma in Pakistani population over
6 years of study period.
Method:
All biopsy specimens diagnosed as ameloblastoma during the
study period (January 2010
–
December 2015) were included in the study.
The slides were reviewed and information pertaining to patient demo-
graphics, clinical presentation and tumour site was recorded on specifi-
cally designed proforma.
Results:
Total 42 cases of ameloblastoma diagnosed during the entire
study period. A wide age range (3 to 80 years) was observed with mean
age 32 years at presentation. Highest incidence was recorded in 20
–
40 year age group. A slight male preponderance was noted (57 %).
Virchows Arch
(
2017
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