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Objective:
Whole slide imaging, converts an entire slide into a digital
image that allows seeing in a computer screen all slide instead of a chosen
area. WSI has been performed in Acibadem University the department of
Pathology since 2013 for routine histopathologic & cytopathologic diag-
nosis. We conducted a validation study regarding the use of WSI based on
an institutional experience.
Method:
Two experienced pathologists were enrolled for the study. One
pathologist was located in Ankara, and the other worked in various AHG
locations. The digital slide scanner used was either Pannoramic Flash 250 or
Pannoramic Midi from 3DHISTECH. They examined the glass slides then
reviewed their former report to note if there were any differences in their
previous diagnoses. The cases were classified as agreement, disagreement-
minor and disagreement-major. For disagreements, pathologists were asked to
describe the reason(s) for it.
Results:
Result for pathologist 1: Agreement between digital and paraffin
was 94.8 %. The 5.2 % disagreement was mostly (4.2 %) due to failure to
detect rare H. Pylori microorganisms. If H.pylori is not considered than
the agreement would be 99 %. Result for pathologist 2: The difference
between digital versus paraffin diagnoses is 2,1 %. Agreement is 97.9 %.
Conclusion:
One software adjustment may not fit for all. Routine pathol-
ogy sign-out sessions (including frozen sections, IHC, FISH, BDISH) may
be performed securely with virtual slides. However more validation studies
might be necessary since the spectrum of pathology specimens is very wide.
OFP-06-001
P57 immunostaining pattern of complete mole in Lagos University
Teaching Hospital
O. Oguntunde
*
, N. Awolola, E. Daramola, F. Abdulkareem, A. Banjo
*
Lagos University Teaching Hospital, Dept. Anatomic and Mol.
Pathology, Nigeria
Objective:
P57 immunohistochemistry has been proven as a veritable
ancillary technique in diagnosis of molar pregnancy especially in differ-
entiating between partial and complete mole (CM). CM does not express
the staining because p57 is paternally imprinted and maternally
expressed. This study is aimed at highlighting the p57 staining pattern
amongst CM diagnosed in our institution
Method:
Formalin Fixed Paraffin Embedded sections from archived
blocks (2008
–
2014) were made for p57 immnuohistochemistry using
auto immunostaining processing according to DAKO guidelines. The
staining waas referred as adequate with positive internal control (decidua,
extravillous trophoblasts) or external control (normal products of concep-
tion). The staining pattern of the cytotrophoblast nuclei and stroma villus
cells were recorded
Results:
One hundred percent of the CM stained negatively for p57 with
75 % having positive internal control. Twenty-five percent of the cases had
no internal control staining positive, however the external control in the
batches were positively stained. The CM seen in our environment were
largely within the 2nd trimester accounting for 90 % of the gestational ages
with majority showing degenerative changes like calcifications
Conclusion:
In conclusion, the pattern of staining of CM amongst African
origin has been found to be comparable with that of developed countries
despite the commoner mid trimester presentation and degenerative changes
OFP-06-002
Novel markers for distinguishing gonadal elements in disorders of
sex development with gonadal dysgenesis
K. Gwin
*
, R. Buell-Gutbrod, A. Montag, M. Reyes-Múgica
*
UT Southwestern Medical Center, Pathology, Dallas, USA
Objective:
To propose a panel of immunohistochemical markers that can
contribute to distinguish male, female and steroid-producing gonadal el-
ements in disorders of sex development (DSD).
Method:
FOXL2 is required for granulosa cell differentiation and
expressed in ovarian stroma. Its counteracting transcription factor
SOX9 is a target of SRYand essential for testis development by formation
and maintenance of Sertoli cells. DMRT1 represses FOXL2 and is nec-
essary to maintain testis differentiation. CYP11A1 and StAR are enzymes
in the testosterone biosynthetic pathway. This panel was applied to 15
cases of dysgenetic gonadal tissue in 10 patients with DSDs.
Results:
In ovo-testis and mixed gonadal dysgenesis, the sex cord-
stromal cells of ovarian type tissue (Granulosa cell lineage) expressed
FOXL2. Streak gonads showed a similar expression pattern of FOXL2
as normal ovarian-type stroma. Sex cord-stromal cells in testicular type
tissue (Sertoli cell lineage) showed nuclear staining of SOX9 and
DMRT1, including Sertoli-cell hamartoma. CYP11A1 and StAR
highlighted the presence of steroid-producing cells.
Conclusion:
Based on the distinct expression of FOXL2, SOX9 and
DMRT1 in dysgenetic gonads, our findings support that this panel is
useful to distinguish male and female gonadal components. CYP11A1
and StAR are useful for identification of steroid-producing cells.
OFP-06-003
Two cases of bilateral hyperplastic perilobar nephroblastomatosis
mimicking Wilms tumour: A case study with serious clinical impacts
T. Micsik
*
, Z. Sápi, M. Csoka, G. Vujanic
*
Semmelweis University, Dept. of Pathology and Exp. Cancer Research,
Budapest, Hungary
Objective:
Nephrogenic rests (NRs) are the precursors of Wilms Tumour
(WT) and have different stages in their life-cycle. Most NRs are micro-
scopic, but hyperplastic NRs can grow expansively and mimic WT.
Method:
Two children with lesions in their kidneys. Patient 1: A 9 month
old male with bilateral, 1
–
6 cm, encapsulated, partially necrotic nodules,
mainly of epithelial structures. Patient 2: A 23 month old female with
multiple, 1
–
4 cm, partly encapsulated nodules of mainly epithelial struc-
tures, with some regressive changes.
Results:
Despite the encapsulation, size and chemotherapy-induced
changes (CIC) of these nodes, the diagnosis of bilateral hyperplastic
nephroblastomatosis was made.
Conclusion:
NRs are more common than we might think and hyperplastic
NRs can mimic WT. Literature data are quite scarce on this topic and based
on USA material, where preoperative chemotherapy is not used, thus lack
the experience of chemotherapy effects on NRs. According to these papers,
the best discriminative feature of WTs is encapsulation, which in fact can
occur in NRs due to preoperative chemotherapy (usually given in Europe).
Based on literature, one can over-diagnose hyperplastic NRs as WT and
give wrong treatment. There is a need for update in literature data on
hyperplastic NRs and their appearances after chemotherapy.
OFP-06-004
Sarcoma with CIC-DUX4 gene fusion: Case report of kidney tumour
location in a 12-year-old boy
H. Sartelet
*
, C. Perret, G. Pierron, A. Mc Leer, C. Piolat, C. Durand, D.
Plantaz
*
CHU Grenoble, Dept. of Pathology, France
Objective:
Recent molecular advances have identified a novel sarcoma
defined molecularly by oncogenic fusion of the genes CIC and DUX4
termed CIC-DUX4 sarcomas. The most common site of involvement was
the trunk but some cases have been described in the head and neck and
extremities. We report one of the first cases of primitive renal CIC-DUX4
sarcoma
Monday, 4 September 2017, 17:15
–
19:15, D203
OFP-06 Paediatric and Perinatal Pathology
Virchows Arch
(
2017
)
471
(
Suppl 1
):
S1
–
S352
S17