ECP 2017 - Abstract Book

The Flemish Renal Biopsy Registry contains clinical and pathological

data including FCGG code from all non-transplant biopsies, starting from

Jan 1st 2017.

Conclusion:

We have designed a renal pathology coding system along

with setting up the Flemish Renal Biopsy Registry. Future efforts will be

made to validate the FCGG system for renal biopsy registration.

OFP-07-010

How successful is an experienced nephropathologist in diagnosing

adult minimal change disease when electron microscopy is not

accessible?

D. Baydar

, T. Yildirim, S. Kaya, B. Gurel, A. Saglam Ayhan

Hacettepe Un. School of Medicine, Pathology, Ankara, Turkey

Objective:

Minimal change nephrotic syndrome is considered as a diag-

nosis of electron microscopy (EM). However, EM facility is not available

in every institution. In this study, we aimed to explore the success rate of

minimal change disease (MCD) diagnosis in the renal biopsies of adult

nephrotic patients in which EM was not performed.

Method:

79 adults which were given possible diagnosis of MCD by one

of 2 experienced nephropathologists between 2000 and 2016 in a single

institution were investigated for their therapy response. Biopsies had been

studied by light and immunofluorescence microscopy without EM. Only

the patients who had

≥

6 months follow-up after immunosuppressive-

therapy were included (

= 43).

Results:

All patients responded to treatment. 38 showed complete where-

as remaining 5 had partial remission. None of the patients progressed to

chronic renal disease. There were no histological differences between

cases showing partial or complete remission. Ratio of globally sclerosed

glomeruli ranged between 0 and 30 %. IF/TA involved

≤

25 % of cortex.

Interstitial inflammation and vascular sclerosis were mild if present.

Conclusion:

Response rate to immunosupressive-treatment in our MCD

patients were similar to other series. This may indicate that diagnosis of

MCD can be suggested reliably by experienced nephropathologists with-

out contribution from EM which may not be accessible due to various

reasons.

OFP-07-011

Clinicopathological correlation of findings in patients with renal and

bone marrow biopsies

G. Basmaci

, B. Sarsik, A. Celtik, N. Ozsan, M. Hekimgil, F. D.

Koseoglu, G. Saydam, H. Toz, S. Sen

Ege University, Pathology, Izmir, Turkey

Objective:

Monoclonal gammopathies (MG) can cause renal impairment

and proteinuria. Amyloidosis, cast nephropathy, light chain deposition

disease (LCDD) and glomerulonephritis can be seen in renal biopsies

secondary to hematological diseases. We aimed to examine the clinico-

pathological findings of the patients having both bone marrow and renal

biopsies.

Method:

Between 2012 and 2015, 1030 patients had obtained renal bi-

opsy in our center. One hundred thirty of them had also bone marrow

biopsy(12.6 %). We reevaluated the bone marrow and renal biopsies of all

cases in the aspect of clinical and laboratuary findings.

Results:

Among 130 cases that had both bone marrow and renal biopsy

40 were diagnosed as multiple myeloma and plasma cell dyscrasia in

bone marrow biopsies. Among these cases, the most common findings

in renal biopsies were AL amyloidosis (57.5 %); cast nephropaty (25 %);

tubular injury (10 %); tubular interstitial nephritis (TIN) (0,2 %); LCDD

(0,2 %). Forty-nine cases were diagnosed as renal amyloidosis (37.7 %).

In bone marrow biopsies of 15 cases that had renal amyloidosis, amyloid

deposition was not identified. Among the renal amyloidosis patients, 23

were evaluated as AL, 12 were AA; 4 were nonAA/AL, one case was

ATTR; 9 cases couldn

’

t be subtyped. AL amyloidosis was the most com-

mon renal morphological finding in our study. We found lower rates of

tubulopathy and LCDD when compared to literature.

Conclusion:

Clinicopathological correlation and awareness of renal man-

ifestations associated with MG are important in the evaluation of renal

biopsies. Data about glomerular pathological findings associated with

monoclonal gammopathies is limited and need further investigations.

OFP-07-012

NCAM and FGFR1 over-expressions are the earliest molecular

changes upon TGF-

induced renal fibrosis in vitro

—

key targets

for further strategies to ameliorate renal fibrosis

M. Zivotic

, B. Tampe, X. Xu, X. Tan, G. Nyamsuren, C. Müller, S. Saito,

M. Zeisberg, G. Mueller, J. Markovic-Lipkovski

Institute of Pathology, Belgrade, Serbia

Objective:

The major challenges are to look deeper into signaling path-

ways driving renal fibrosis and to define key molecular changes underling

such process.

Method:

TGF-

-induced epithelial-mesenchymal transition (EMT) of

kidney tubular epithelial cells (HK-2 cell line) was used as an established

in vitro model of renal fibrosis. HK-2 cells were seeded in 6-well plate

divided in control, TGF-

treated and TGF-

+ FGFR inhibitor

(PD173074) treated groups. EMT changes were followed optico-micro-

scopically, using immunolabeling and qRT-PCR, following dynamical

changes of gene expression.

Results:

TGF-

induced EMT was morphologically clearly visible after

72 h. However, molecular changes characteristic for EMT were detected

earlier: 48 h after TGF-

treatment relative mRNA levels of SLUG,

SNAIL, TWIST, MMP2, MMP9, N-cadherin, integrin-

-SMA and

FSP-1 were significantly up-regulated and E-cadherin was down-

regulated in TGF-

group (

< 0.001). Even more, NCAM and FGFR1

molecules achieved their pick of over-expression 24 h after EMT induc-

tion. These changes revealed completely new approach to our further

experiments and lead us to try to inhibit EMT events by administration

of PD173074. Surprisingly, PD173074 restores all EMT induced changes

including morphology and molecular characteristics.

Conclusion:

NCAM and FGFR1 were the earliest over-expressed gens

upon TGF-

induced EMT of HK-2 cells and PD173074 almost

completely prevented TGF-

induced EMT, suggesting potential of

PD173074 to ameliorate renal fibrosis.

OFP-08-001

Prognostic significance of tumour-infiltrating lymphocytes according

to molecular subtype in breast cancer patients who received adjuvant

chemotherapy

Y.-K. Bae

, H.-J. Kwon, N. Jang, M.-H. Park

Yeungnam University, Dept. of Pathology, Daegu, Republic of Korea

Objective:

This study investigated prognostic value of tumour-

infiltrating lymphocytes (TILs) according to molecular subtype of inva-

sive breast cancer (IBC) in patients who received adjuvant chemotherapy.

Method:

TILs were evaluated in 1,269 IBCs using the standard method

and the cases were classified into high and low TILs groups based on a

10 % cutoff. Correlations of TILs with clinicopathological characteristics

and prognosis were investigated.

Results:

Of the 1,269 IBC patients, 388 (30.6 %) had high TILs and 881

(69.4 %) had low TILs. High TILs was associated with ER and PR

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