ECP 2017 - Abstract Book

Conclusion:

Conduction system should be thoroughly investigated in

cases of calcific aortic valve stenosis. The results warrant further investi-

gation in this field.

E-PS-03-005

Histopathological differences between hypertrophic and dilated

cardiomyopathy

P. Tsiviltidou

, E. Chatzifotiou, A. Nikolaou, I. Dimitriadis, G. Tagarakis,

D. Anestakis

Aristotle University Thessaloniki, Dept. of Histopathology and Forensic

Medicine, Greece

Objective:

Hypertrophic Cardiomyopathy (HCM) is an inherited disor-

der characterized by the presence of increased left ventricular wall thick-

ness. Dilated Cardiomyopathy (DCM) develops when the ventricles en-

large and weaken causing contractile dysfunction of the cardiac muscle.

Method:

In order to define the HCM-DCM differences, histological and

immunohistochemical examination of left ventricular tissue fragments

was performed. The stains used for histological examination were

Hematoxylin&Eosin and Masson

’

s Trichrome(TRI), whereas the anti-

bodies used for the immunohistochemical study were Anti-Desmin,

Anti-CD34, Anti-Collagen I and Anti-TnT.

Results:

H&E staining in DCM revealed slightly enlarged or wavy, elon-

gated muscle fibers with hypochromatic, heterogeneous vascular sarco-

plasm, by a decrease of myofibril numbers. In HCMmyofibrillar disarray

and disorganization were observed, combined with myocytes enlarge-

ment and hyperchromatic nuclei. Immunohistochemical expression and

staining patterns of the markers Desmin, TnT and CD34 confirmed the

above findings, with CD34 being a specific diagnostic marker for DCM.

TRI and H&E indicated in both CMPs dense collagen fibrils with the

interstitial tissue in DCMto be quadrupled. Immunostaining with Col I

corroborated this increased fibrotic process.

Conclusion:

Numerous features differentiate DCM from HCM with

myocardial size and architecture being predominant. Interstitial fibrosis

in DCM surrounds and isolates myocytes, while in HCM forms foci.

DCM also increases T-lymphocytes number.

E-PS-03-006

On pathological neuro-vascular factors of idiopathic and renal

hypertension

E. Neu

, M. C. Michailov, U. Welscher, T. Senn, M. Traub, H. Dahlheim,

J. Foltinova, G. Werner, G. Weber

Inst. Umweltmed. c/o ICSD e.V., Munich, Germany

Objective:

Since discovery of blood circulation (HARVEY 1628),

homoeostasis of blood-pressure (CANNON) & blood volume

(GAUER) are till today genuine and renal hypertension pathogenesis incl.

apoplexy cerebri (HIPPOCRATES) not clarified.

Method:

Vascular motor activity, rat-blood-pressure = BP. Ref.: 1. author.

Handout:

Results:

Isolated prep.: Angiotensin-II/5-HT/PGs/vasopressin = VP & K+

induce spontaneous-phasic (SPC:0.5

–

2/min) in rat portal/human renal veins

(high sensitivity to Angiotensin-II-contr. to 50 pg/ml), but periodic slow-tonic

contractions (STC: 0.1

–

0.2/min) in rat-aorta/human renal&uterine arteries.

BP-observations: Transformation of depressor response to

acetylcholine&electrical-central-vagal stimulation (CVS:55Hz,2ms,5 s,5 V)

into biphasic depressor-pressor=dR/pR by nicotine & mercptoethylguanidine

(MEG: inhibitor of NO-synthase), also pR-potentiation from VP (5

–

100 mIU), non-/AHR-600/McN-A-343 & nicotine-like/DMPP: 0.1

–

100 mg/kg ganglion-stimulating agents, inverting serotoninergic dR. OPEN

QUESTIONS 1. Existing of correlation between STC and low-frequency

fluctuations (Mayer-waves) & pericytes? 2. Appearance of SPC/STC in hu-

man vascular prep. from patients/surgical tissue with hypertonia genuina et

renalis, atherosclerosis? 3. Importance of phenomena A&BP for angio-cardi-

ac/cerebral spasms: infarct & apoplexy?

Conclusion:

Drugs, endogenic substances & psychosomatic factors could

sensitize BP regulatory central, spinal, neural & peripheral structures caus-

ing idiopathic hypertension, leading probably to angiocardiac cerebral

spasms via biphasic dR/pR or augmented pR by endogenic or exogenic

factors. Further observations could help for new therapies in context of

UNO-Agenda21 for better health, education, etc. on global level.

E-PS-04-001

Metastasis of colon cancer to the thyroid gland: A case diagnosed on

fine-needle aspirate by a combined cytological, and immunocyto-

chemical approach

Z. Yildirim Ekin

, U. Aykutlu, A. Veral, D. Nart

Ege University Faculty of Medicine, Dept. of Pathology, Izmir, Turkey

Objective:

Metastasis of colon cancer to the thyroid gland is extremely

rare in routine clinical practice. Therefore, it is often not included in the

differential diagnosis of patients presenting with malignant thyroid cytol-

ogy. We report a case of primary colon carcinoma metastasis to the thy-

roid with cytological features.

Method:

This rare case is re-evulated on the basis of the literature

Results:

66-years-old female patient has presented with 1.5 cm firm, solid

nodule in the left thyroid lobe. FNA was performed and cell block was

prepared by the plasma-thrombin clot technique. Three years ago, she

underwent a sigmoid colectomy for adenocarcinoma. On both Giemza

and Papanicolau stained smears, abundant cell necrosis was evident; besides

necrosis, epithelial cell population showed cells with irregular contours,

nuclei were elongated,

“

cigarlike

”

shaped, with coarse granular chromatin,

which had not reminded primary thyroid carcinoma. Cell block showed that

similar morphological features. The cell groups revealed architectural ab-

normalities with a high degree of cell overlapping; and cribriform

arrengement. At higher magnification, cellular atypia was evident; cyto-

plasms Tumour cells were positive for CK20, CDX2, and negative for

TTF-1 and CK7. The case was reported as colon adenocarcinoma metastasis

Conclusion:

We were presented this case to kept in mind metastases

when examining thyroid FNA

E-PS-04-003

The heat induced antigen retrieval has influence on immunocyto-

chemistry results in alcohol-fixed cell samples

K. Cizkova

, P. Flodrova, J. Ehrmann

Palacky University Olomouc, Czech Republic

Objective:

Immunohistochemistry (IHC) and immunocytochemistry (ICC)

play irreplaceable role in diagnostic. It is well known that antigen retrieval

technique has beneficial outcomes on IHC results on formalin-fixed, parafin-

embeded tissue samples. The main purpose of antigen retrieval is breaking

protein crosslinks which are formed during formalin fixation. It also provides

additional advantages, such as use of higher antibody dilution and increase

the staining specificity. On the other hand, cytological samples are fixed in

alcohol-based fixatives which does not leads to formation of crosslinks thus

antigen retrieval technique does not seem to be necessary step in ICC.

Method:

Alcohol-fixed HEK293 cell line and patient cytological sam-

ples from thyroid gland obtained by fine needle aspiration (FNA) tech-

nique were used in this study. We compared indirect two-step ICC stain-

ing results performed according to protocol with or without heat induced

antigen retrieval for several antibodies.

E-PS-04 Cytopathology

Virchows Arch

(

2017

)

471

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Suppl 1

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S352

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