ECP 2017 - Abstract Book

of 62 NSGCT/mixed GCT (73.7 % displaying teratomatous, 52.6 %YSD

components). GCTwith YSD/GPY3+ (

= 26) displayed more CTR, but

not significantly compare to cases without YSD/GPY3+ (

= 18). Mixed

GCT (

= 17) associated significantly (

= 0,001) with CTR compare to

NSGCT (

= 23).

Conclusion:

Our results corroborate that GPY3 increases diagnostic ac-

curacy of YSD. Mixed GCT (e.g, with teratomatous component) is an

important predictor of CTR in testicular GCT.

OFP-09-012

Correlation between expression of epigenetic silencer EZH2 and

Claudin-4 and their prognostic value in urothelial bladder cancer

—

immunohistochemical study

S. Stojnev

, M. Krstic, A. Ristic, J. Todorovic, M. Mladenovic, A. Ristic-

Petrovic, I. Conic, L. Jankovic Velickovic

Faculty of Medicine, Dept. of Pathology, Ni

, Serbia

Objective:

Ezh2 transcriptional repressor has a major role in oncogenesis

of the bladder. Since epigenetic mechanisms are crucial in alteration of

Claudin-4 in urothelial bladder cancer (UBC), we investigated the possi-

ble correlation between Ezh2 and Claudin-4 expression and their prog-

nostic impact.

Method:

We analyzed the relationship between immunohistochemical

expression of Ezh2 and Claudin-4 in 588 cases of UBC (182-Ta, 279-

T1, 127-T2 tumours), included in tissue-microarrays, and clinicopatho-

logical data.

Results:

High nuclear expression of Ezh2 was strongly associated with

high tumour grade, advanced stage, carcinoma in situ (

< 0.001, respec-

tively), and variant differentiation of UBC (

= 0.035). Moreover, Ezh2

was linked to hematuria and cancer specific death (

= 0.031, and

< 0.001, respectively). Ezh2 and membranous Claudin-4 staining cor-

related directly (

< 0.001). Claudin-4 exhibited association with tumour

grade, stage, and recurrent disease (

< 0.001,

= 0.001,

= 0.008,

respectively). Kaplan-Meier analyses showed that high Ezh2 expression

predicted worse survival of the patients (

< 0.001), while Claudin-4

indicated shorter recurrence free survival (

= 0.034).

Conclusion:

Correlation of high Ezh2 expression and strong Claudin-4

staining may suggest that Ezh2 activity has limited influence to Claudin-4

regulation. Overexpression of both Ezh2 and Claudin-4 indicates aggres-

sive behavior of UBC. They may therefore serve as useful biomarkers for

UBC and promising targets for therapy.

OFP-10-001

Immunohistochemical evaluation of the phosphorylated AKT1 ex-

pression in well-differentiated pancreatic neuroendocrine tumours

V. Delektorskaya

, O. Solovieva, G. Chemeris, Y. Patyutko

Russian Cancer Research Centre, Dept. of Pathology, Moscow, Russia

Objective:

The Akt signaling pathway is involved in tumour cell prolif-

eration and survival. We aimed to determine phosphorylated AKT1

(pAKT1) immunoexpression in a cohort of well-differentiated pancreatic

neuroendocrine tumours (PNETs).

Method:

pAKT1 status of the tumours was analyzed using an immuno-

histochemical analysis (IHC). Expression levels were associated with

tumour characteristics and clinical outcomes.

Results:

Forty-one patients (21 males, median age 57 years) with PNET

Grade (G) 1 (

= 12), 2 (

= 20), 3 (

= 9) were studied. Nuclear

expression of pAkt1 was detected in 8.3 % G1, 45.0 % G2, and

88.9 % G3 pNET cases. Overexpression of pAKT1 was observed most

frequently in primary and metastatic pNETs G3. No correlation was

found between pAkt1 expression and tumour size, lymphovascular inva-

sion, somatostatin receptor 2A status, overall survival.

Conclusion:

pAKT1 is observed in different groups of pNET patients with

the highest expression levels in well-differentiated PNETs G3. The associa-

tion of pAKT1 to enhanced aggressiveness and histological grade suggests its

potential value as prognostic and predictive biomarker and target in PNETs.

OFP-10-002

Is it about time that liquid-based preparation substituted for conven-

tional smear in thyroid fine needle aspiration? A systematic review

and meta-analysis

Y. Chong

, S.-J. Ji, C.-S. Kang, E.-J. Lee

Catholic University of Korea, St. Mary

’

s Hospital, Dept. of Pathology,

Seoul, Republic of Korea

Objective:

Conventional smear (CS) using fine needle aspiration cytol-

ogy (FNAC) has been established as the test of choice for diagnosing

thyroid lesions, despite low sample adequacy and inter-individual varia-

tions. Although a liquid-based preparation (LBP) technique has been

recently applied to overcome these limitations, its clinical utility and its

accuracy over CS is controversial. This study aimed to determine the true

sensitivity and specificity of LBP in thyroid FNAC by meta-analysis.

Method:

We searched major electronic databases (MEDLINE, Embase,

Cochrane library, Google Scholar) with queries of

“

thyroid,

” “

liquid-

based preparation,

”

and

“

liquid-based cytology.

”

Original articles includ-

ing cytohistologic correlation data comparing the accuracy of any LBP

technique, such as ThinPrep, SurePath, and Liqui-Prep, with CS were

included for qualitative meta-analysis and preparation of synthesized re-

porter operating curves (sROC).

Results:

A total of 372 studies were screened and 51 original articles were

eligible for full-text review; finally, 24 studies were chosen for the meta-

analysis. Average sample inadequacy was significantly lower in two main-

stream LBP methods (ThinPrep and SurePath) than CS. Specificity and

sensitivity by sROC were similar or slightly superior for LBP versus CS.

Various cytomorphologic changes by each method have been reported.

Conclusion:

Although a learning curve is essential for adapting to the

cytomorphologic features of the LBP technique, our results support the

use of two mainstream LBPs alone in thyroid FNAC that LBP will in-

crease the sample adequacy and reduce the workload with similar accu-

racy. More data and further evaluation are needed for the other LBPs to

confirm they provide similar results.

OFP-10-003

Incidence of non-invasive follicular thyroid neoplasm with papillary-

like nuclear features (NIFPT) in a series of papillary thyroid carci-

noma (PTC)

M. R. Bella-Cueto

, A. Petit-Montserrat, N. Baixeras González, A. León-

Castillo, N. Combalia Soriano, I. Català Costa, R. Orellana Fernández, C.

Padilla Navas, I. Capel Flores, V. Perez Riverola, S. Barcons Vilaplana, X.

Guirao Garriga, P. Moreno Llorente, M. E. De Lama Salvador, J. Puig De

La Bellacasa, M. Rigla Cros, X. Matias-Guiu, J. C. Ferreres Piñas

Hospital Parc Taulí, Pathology, Sabadell, Spain

Objective:

To determine the clinical, cytological and follow-up charac-

teristics of NIFPT in a retrospective series of PTC.

Method:

Subjects: patients operated in two institutions between 2005 and

march 2017 with histological diagnosis of non-invasive follicular variant

of PTC or follicular-patterned neoplasm of uncertain malignant potential

(FPN-UMP) due to their nuclear characteristics. Method: retrospective

observational study. Variables collected: age, sex, previous cytological

Tuesday, 5 September 2017, 17:15

–

19:15, G109

OFP-10 Joint Session: Endocrine Pathology / Infectious

Diseases Pathology

Virchows Arch

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2017

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