46 / 366
Method:
We studied 10 pituitary glands of 4 female and 6 male (the average
age was 56,5 ± 4,8 years) with cardiovascular and oncological diseases. We
used the Gordon and Sweet
’
s silver staining method, double staining immu-
nohistochemistry with 11 hormone combinations, confocal laser scanning
microscopy (CLSM) with a mixture of 5 hormones. These combinations
were: prolactin /thyroid-stimulating hormone (TSH), prolactin/luteinizing
hormone (LH), prolactin /follicle-stimulating hormone (FSH), prolactin/
adrenocorticotropic hormone (ACTH), growth hormone (GH)/TSH, GH/
LH, GH/FSH, GH/ACTH, TSH/LH, TSH/FSH, TSH/ACTH.
Results:
We found that the same cells of the normal adenohypophysis can
co-express prolactin with ACTH, TSH, FSH, LH; GH with ACTH, TSH,
FSH, LH, and TSH with ACTH, FSH, LH. The comparison of the aver-
age co-expression coefficients of prolactin, GH and TSH with other hor-
mones showed that the TSH co-expression coefficient was significantly
the least (9,5 ± 6,9 %; 9,6 ± 7,8 %; 1,0 ± 1,3 % correspondingly).
Conclusion:
Plurihormonality of normal adenohypophysis is an actually
existing phenomenon, which refutes the concept
“
one cell type
—
one
hormone
”
, as had been accepted before.
OFP-11-001
Clinical relevance of histological grading based on poorly differenti-
ated clusters (PDC) in patients with rectal carcinoma treated with
neo-adjuvant chemo-radiotherapy
V. Barresi
*
, S. Lionti, F. Domati, L. Reggiani Bonetti
*
University of Messina, Dept. of Human Pathology, Italy
Objective:
The clinical outcome of patients with locally advanced rectal
cancer after neo-adjuvant chemo-radiotherapy (CRT) depends on tumour
response to treatment that can be measured through tumour regression
grade (TRG) and post-treatment (y) TNM stage. Currently, little is known
on the prognostic relevance of pre-treatment histopathological features of
rectal cancer. In this study we aimed to investigate the prognostic value of
histological grading based on the counting of poorly differentiated clus-
ters (PDC) of neoplastic cells in pre-treatment biopsies of rectal cancer
submitted to neo-adjuvant CRT.
Method:
Grading based on PDC counting was retrospectively applied to
204 pre-treatment endoscopic biopsies of rectal carcinomas treated with
neo-adjuvant CRT and surgery.
Results:
. Inter-observer agreement in the assessment of PDC grade was
good (K: 0,79). High PDC grade was significantly associated with high
yTstage (
P
= 0,044), yM+ status (
P
= 0,0004) and unchanged TNM stage
or TNM upstaging (
P
= 0,032). In addition, it was a significant and
independent prognostic factor for cancer specific survival (CSS).
Conclusion:
Pre-treatment high PDC grade is significantly associated
with low response to therapy and worse prognosis. This suggests that it
might be used to discriminate potential non-responders to neo-adjuvant
CRT and to design tailored therapeutic strategies for patients with locally
advanced rectal cancer.
OFP-11-002
SERPINB5 overexpression and its association with CCRT resistance
and prognostic importance in rectal cancers
I.-W. Chang
*
, C.-F. Li
*
E-DA Hospital, Dept. of Pathology, Kaohsiung, Taiwan
Objective:
Due to the varying characteristics and conflicting outcomes
on overall survival of rectal cancers (RCs), many studies have been un-
dertaken to determine various prognostic and predictive factors for its
mainstay treatment of CCRT followed by surgery. Cell motility of cancer
cells contributes to tumour invasion, migration and eventually metastasis.
However, the genes associated with cell motility (GO:0048870) had yet
been systemically evaluated in RCs.
Method:
A comparative analysis of gene expression profiles was applied
to a transcriptomic dataset (GSE35452) with focus on genes associated
with cell motility, where SERPINB5 was recognized as the most signif-
icantly upregulated. 172 primary RCs which underwent neoadjuvant
CCRT f o l l owe d b y s u r g i c a l r e s e c t i o n we r e c o l l e c t e d .
Immunohistochemical study was used to semiquntatively assess the ex-
pression level of SERPINB5 protein.
Results:
High immunoreactivity of SERPINB5 was significantly linked
to pre- and post-CCRT advanced disease, lymphovascualr invasion, and
poor response to CCRT (all P
≤
0.015). SERPINB5 overexpression was
not only negatively associated with disease-specific (DSS), local
recurrence-free (LRFS) and metastasis-free survival (MeFS) rates in uni-
variate analysis, but also an independent prognostic factor for DSS and
MeFS in RCs (all P
≤
0.043).
Conclusion:
SERPINB5 may play an important role in RC progression
and response to neoadjuvant CCRT, and serve as a novel prognostic factor.
OFP-11-003
Increased homogeneity and interobserver agreement after addition
of p53 staining within a digital expert panel for Barrett
’
s oesophagus
M. van der Wel
*
, R. E. Pouw, K. A. Seldenrijk, G. J. A. Offerhaus, M.
Visser, F. J. W. ten Kate, K. Biermann, M. Doukas, C. Huysentruyt, A.
Karrenbeld, G. Kats-Ugurlu, J. S. van der Laan, I. van Lijnschoten, F. C.
Moll, A. H. Ooms, H. van der Valk, J. G. Tijssen, J. J. Bergman, S. L. Meijer
*
Academ. Medisch Centrum Amsterdam, Dept. of Pathology, Dept. of
Gastroenterology & Hepatology, The Netherlands
Objective:
Interobserver agreement for dysplasia in Barrett
’
s oesophagus
(BE) is low and guidelines advise expert review of dysplastic cases.
Therefore, a digitalized review platform was set up in the Netherlands,
employing 5 core BE pathologists and expanded with 10 other BE pa-
thologists to reach extended coverage. We assessed the added value of
p53-IHC on the assessment of neoplastic BE.
Method:
Sixty single HE slide BE cases (20 NDBE, 20 LGD and 20
HGD) were digitalized and independently assessed by 9 BE pathologists.
After wash-out time, cases were re-assessed with the addition of concor-
dant p53-IHC slides. Outcomes were: number of IND diagnoses, inter-
observer agreement and accuracy of the 9 BE pathologists compared to
gold-standard diagnosis.
Results:
Addition of p53-IHC decreased the mean number of IND diag-
noses from 10/60 to 7/60 (
p
= 0.08). Mean interobserver agreement in-
creased significantly from 0.62 to 0.77 (dysplasia versus no dysplasia,
p
= 0.0001). Accuracy compared to the GS diagnosis increased signifi-
cantly from 80 to 88 % (
p
= 0.003).
Conclusion:
Addition of p53-IHC significantly improves homogeneity
within the BE review panel, increases interobserver agreement and accura-
cy; and decreases the number of IND diagnoses. This can ultimately lead to
a lower number of endoscopies and better standard of care for BE patients.
OFP-11-004
Assessment of tumour budding in lymph node and distant metastases
of stage IV colorectal cancer patients
A. Blank
*
, S. Burren, I. Zlobec, H. Dawson, A. Lugli
*
University of Bern, Institute of Pathology, Switzerland
Objective:
Tumour budding (TBD) is an additional prognostic factor in
colorectal cancer (CRC) based on the UICC TNM classification 2017.
Data on TBD In lymph node (LN) and distant metastases (DM) in com-
parison to primary CRC are still missing.
Wednesday, 6 September 2017, 08:30
–
12:00, G104-G105
OFP-11 Digestive Diseases Pathology - GI
Virchows Arch
(
2017
)
471
(
Suppl 1
):
S1
–
S352
S32