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E-PS-12-016
Sclerosing polycystic adenosis of parotid: A rare entity
S. Ben Rejeb*, A. Blel, R. Aloui, M. Ksentini, Y. Zidi, S. Ben Khalifa, A.
Sassi, E. Kazdaghli, R. Ayadi, R. Lahiani, M. Ben Salah, N. Znaidi, S.
Rammeh
*Security Forces Hospital, Dept. of Pathology, Tunis, Tunisia
Objective:
Sclerosing polycystic adenosis(SPA) is a rare inflammatory
condition of the salivary glands characterized by fibrocystic changes and
adenosis. Less than 60cases have been described in literature. We aim to
report a case of SPA of the parotid gland with a brief review of literature.
Method:
A 68-year old woman presented with a 4 months history of a
painful mass of the parotid. On examination there was a well-defined,
1,5 cm mobile and firm mass of the right parotid. A fine needle aspiration
biopsy specimen concluded to pleomorphic adenoma. Superficial
parotidectomy was performed.
Results:
On gross examination, the specimen measured 4 × 2x1cm and
contained a 1 cm well-circumscribed unencapsulated nodule with
inverted core. Intraoperative frozen-section concluded to benign lesions.
Microscopic examination revealed a sharply circumscribed
unencapsulated and lobulated nodule composed of acini, ducts and
cribrifomr structures covered with round cells showing regular nuclei.
Focally the cells exhibit large abundant eosinophilic cytoplasmic gran-
ules. The stroma was fibrous, hyalinized, harbouring an inflammatory
infiltrate. Myxoid changes were observed. On immunohistochemistry,
myoepithelial cells were detected using smooth-muscle-actin. At
3 months follow-up, the patient was free of local recurrence.
Conclusion:
It remains unclear whether SPA of the salivary gland is an
inflammatory pseudotumoural lesion or a neoplastic entity. Although, no
carcinomatous degeneration has been reported yet, long-term follow up is
advised.
E-PS-13-001
Morphometric characteristics of reparative, neoplastic and cancer
spheroid epithelial-mesenchymal structure in cervical biopsy
E. Kogan*, T. Demura
*Setchenov Moscow Med. University, Dept. of Anatomic Pathology,
Russia
Objective:
We evaluated the morphometric characteristics of reparative,
neoplastic and cancer SEMS in biopsy with HPV associated uterine cer-
vical cancerogenesis.
Method:
Samples of 54 patients with uterine pathologies (15 chron-
ic cervicitis, 12 L-SIL, 15 H-SIL and 12 carcinoma) were obtained
and studied histological. Materials were fixed with 10 % formalin
solution, parafinized then microtomed horizontally to thickness
4
μ
m. Parafinized histological samples were stained with H/E and
studied immunohistochemically, with mAb against OCT4. Then
measured area of different types of SB using the program cellSens
Standard.
Results:
OCT4 positivity appeared generally among SEMS. By sizes and
histological characteristics, SEMS classified into three types: reparative,
neoplastic and Cancer. The average area of reparative SEMS was
1141.1 ± 305.0 mkm2, the average area of neoplastic SEMS was
2553.9 ± 656.2 mkm2, the average area of cancer SEMS was
2553.9 ± 656.2 mkm2. Reparative SEMS distributed frequently among
chronic cervicitis and L-SIL samples, while neoplastic SEMS showed
significant positivity among the samples of L-SIL and H-SIL, cancer
SEMS among carcinoma samples with distinguishable atypical polymor-
phic nucleus.
Conclusion:
Different types of SEMS have their morphometric
characteristicsas well as immunohistochemical and, which may be used
for differential diagnosis of HPVassociated uterine cervical pathologies.
E-PS-13-002
Estrogen and progesterone positive primary adenocarcinoma of the
lung: A case report and review of the literature
L. Zakhartseva*, M. Dyatel, M. Yanovytska, O. Guz, I. Zakhartsev
*Kiev City Oncology Center, Dept. of Pathology, Ukraine
Objective:
We report a case of primary adenocarcinoma of the
lung with positive ER, PR expression (which can be observed
in 4-11 % of all lung adenocarcinomas due to different authors).
Method:
A 47-year old woman presented with tumour masses in proxi-
mal bronchus (4,5 cm), pleura and vertebrae. Biopsy from lung and pleura
was performed.
Results:
Microscopically in the lung and pleura there was tumour
of similar histological pattern (adenocarcinoma). It was CK7, ER,
PR positive, HER2 negative. Results of IHC study mislead the
pathologist and the diagnosis was made in favor of breast carci-
noma. Patient started to receive hormone therapy, but after thor-
ough clinical examination a tumour mass in mammary gland was
not found. Further investigation was made and tumour turned out
to be TTF1 positive. Treatment was modified as for lung carci-
noma and resulted in partial response (follow-up for 1,5 year).
Conclusion:
ER,PR positive primary adenocarcinoma of lung can
be a diagnostic challenge for pathologists. ER, PR positive tu-
mour should be TTF negative thus to exclude lung origin.
Probably, such tumours have better prognosis compared with
ER,PR negative primary lung adenocarcinomas. Further investiga-
tion should be performed.
E-PS-13-004
Matrix-associated laser desorption/ionisation (MALDI) mass-
spectrometry imaging in a study of IgA nephropathy linked to
IgG4-sclerosing diseases
M. Ivanova*, F. Magni, O. Dyaydyk, A. Smith, M. Stella, M. Galli, C.
Chinello, V. Denti
*Universita Milano-Bicocca, Dipto. di Medicina e Chirurgia, Monza,
Italy
Objective:
IgG4-related kidney disease (IgG4-RKD) is an autoimmune
systemic disorder characterized by dense infiltration of IgG4-positive
plasma cells in kidneys, but can also manifest in multiple organs with
their enlargement. IgG4-RKD often coexists with other glomerular dis-
eases, such as mesangioproliferative glomerulonephritis or IgA-
nephropathy (IgA-N), which is the most common primary glomerular
disease worldwide. In addition, the course of the disease may be compli-
cated by the presence of tubule-interstital nephritis (TIN). Proteomics and
in particular Matrix-associated laser desorption/ionization (MALDI)
mass-spectrometry imaging (MSI) has already been employed in the
study of IgA-N by our group, detecting specific tissue markers. The aim
of this study is to find specific overlapping proteins with IgG4-RKD,
focusing on disease progression and possible application of the results
in pathology.
Method:
Kidney biopsies with confirmed diagnosis of IgA-N are
analysed by MALDI-MSI. Immunohistochemical IgG4 staining, descrip-
tion of TIN, if present, and evaluation of increased IgG4 level in serum of
these patients will also be performed.
Results:
MSI data are currently under statistical elaboration and will be
presented and discussed.
E-PS-13 Molecular Pathology
Virchows Arch
(
2017
)
471
(
Suppl 1
):
S1
–
S352
S336